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README.md

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# Rhapso
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This is the official code base for **Rhapso**, a modular Python toolkit for the alignment and stitching of large-scale microscopy datasets.
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This is the official code base for **Rhapso**, a modular Python toolkit for stitching (alignment and fusion) large-scale microscopy datasets.
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[![License](https://img.shields.io/badge/license-MIT-brightgreen)](LICENSE)
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[![Python Version](https://img.shields.io/badge/python-3.10-blue.svg)](https://www.python.org/downloads/release/python-3100/)
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## Summary
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Rhapso is a set of Python components used to register, align, and stitch large-scale, overlapping, tile-based, multiscale microscopy datasets. Its stateless components can run on a single machine or scale out across cloud-based clusters.
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Rhapso is a set of Python components used to register, align, and fuse large-scale, overlapping, tile-based, multiscale microscopy datasets. Its stateless components can run on a single machine or scale out across cloud-based clusters.
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Looking forward, we are developing an automated QC system for alignment.
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Rhapso is published on PyPI.
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Rhapso was developed by the Allen Institute for Neural Dynamics.
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Rhapso is developed by the Allen Institute.
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<br>
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```
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Rhapso/
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└── Rhapso/
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├── data_prep/ # Custom data loaders
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├── detection/
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├── evaluation/
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├── affine_fusion/
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├── multiscale/
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├── image_split/
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├── matching/
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├── pipelines/
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│ └── ray/
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│ ├── aws/
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│ │ ├── config/ # Cluster templates (edit for your account)
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│ │ └── alignment_pipeline.py # AWS Ray pipeline entry point
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| | └── fusion_pipeline.py # AWS Ray pipeline entry point
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│ ├── local/
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│ │ └── alignment_pipeline.py # Local Ray pipeline entry point
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| | └── fusion_pipeline.py # AWS Ray pipeline entry point
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│ ├── param/ # Run parameter files (customize per run)
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│ ├── interest_point_detection.py # Detection pipeline script
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│ ├── interest_point_matching.py # Matching pipeline script
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│ └── solver.py # Global solver pipeline script
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│ └── affine_fusion.py # Affine fusion pipeline script
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│ └── multiscale.py # Multiscale pipeline script
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├── solver/
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└── visualization/ # Validation tools
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└── rhapso/
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├── data_prep/ # Data readers and XML/DataFrame preparation
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├── detection/ # Difference-of-Gaussian interest point detection
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├── matching/ # RANSAC-based interest point matching
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├── solver/ # Global optimization and transform solving
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├── affine_fusion/ # Affine fusion
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├── multiscale/ # Multiscale OME-Zarr pyramid generation
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├── split_dataset/ # Dataset splitting utilities
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├── evaluation/ # QC and visualization helpers
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├── util/ # Miscellaneous XML/QC/Neuroglancer utilities
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└── pipelines/
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└── ray/
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├── aws/ # AWS Ray cluster entry points and config templates
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├── local/ # Local Ray entry points
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├── param/ # Example/template YAML parameter files
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├── interest_point_detection.py
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├── interest_point_matching.py
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├── solver.py
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├── affine_fusion.py
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├── multiscale.py
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└── split_dataset.py
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```
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For example:
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- `Rhapso/pipelines/ray/local/alignment_pipeline.py` (local)
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- `Rhapso/pipelines/ray/aws/alignment_pipeline.py` (AWS/Ray cluster)
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- `Rhapso/pipelines/ray/local/fusion_pipeline.py` (local)
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- `Rhapso/pipelines/ray/aws/fusion_pipeline.py` (AWS/Ray cluster)
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3. **Point it to your param file**
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Update the `with open("...param.yml")` line so it reads your own parameter YAML.
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| Environment | Resources | Avg runtime |
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|:----------------------|:---------------------|:-----------:|
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| Local single machine | 10 CPU, 10 GB RAM | ~120 min |
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| AWS Ray cluster | 560 CPU, 4.4 TB RAM | ~30 min |
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| AWS Ray cluster | 560 CPU, 4.4 TB RAM | ~10 min |
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<br>
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*Actual times vary by pipeline components, dataset size, tiling, and parameter choices.*

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