Query about median_feature_CN

[at10101]

Hi,
I would be grateful for advice on how to interpret the median_feature_CN metric in the feature_basic_properties output.
Is this value normalised for coverage, and can it be used to compare ecDNA quantity between samples (for example, for the same amplicon across replicates), or is it only meaningful within a single sample?
Thanks

[jluebeck]

Hi, yes - the median CN (and other CN values are normalized against coverage. It is a fair choice for comparison across replicates.

Thanks,
Jens