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| 1 | +# libraries |
| 2 | +library(ggpubr) |
| 3 | +library(here) |
| 4 | +library(effectsize) |
| 5 | +library(ggrepel) |
| 6 | +library(ggtext) |
| 7 | +library(MASS) |
| 8 | +library(patchwork) |
| 9 | +library(rstatix) |
| 10 | +library(tidyverse) |
| 11 | +library(vegan) |
| 12 | + |
| 13 | +# set path to code sub dir |
| 14 | +setwd(here()) |
| 15 | + |
| 16 | +# import data |
| 17 | + |
| 18 | +df <- read_csv("data/outputs/topology.csv") %>% |
| 19 | + dplyr::select(-c(richness)) |
| 20 | + |
| 21 | +dep_vars <- as.matrix(df[2:ncol(df)]) |
| 22 | + |
| 23 | + |
| 24 | +fit <- manova(dep_vars ~ model, data = df) |
| 25 | +summary(fit) |
| 26 | + |
| 27 | +#get effect size |
| 28 | +effectsize::eta_squared(fit) |
| 29 | + |
| 30 | +post_hoc <- lda(model~., df) |
| 31 | +post_hoc |
| 32 | + |
| 33 | +# plot |
| 34 | +plot_lda <- data.frame(model = df$model, |
| 35 | + lda = predict(post_hoc)$x) |
| 36 | + |
| 37 | +plot_arrow <- as.data.frame(post_hoc[["scaling"]]) %>% |
| 38 | + mutate(var = str_replace(row.names(.), "dep_vars", ""), |
| 39 | + lda.LD1 = scale(LD1), |
| 40 | + lda.LD2 = scale(LD2)) |
| 41 | + |
| 42 | +ggplot(plot_lda) + |
| 43 | + geom_point(aes(x = lda.LD1, |
| 44 | + y = lda.LD2, |
| 45 | + colour = model), |
| 46 | + size = 3, |
| 47 | + alpha = 0.3) + |
| 48 | + #= geom_segment(data = plot_arrow, |
| 49 | + #= aes(x = 0, |
| 50 | + #= y = 0, |
| 51 | + #= xend = lda.LD1, |
| 52 | + #= yend = lda.LD2)) + |
| 53 | + #= geom_text_repel(data = plot_arrow, |
| 54 | + #= aes(label = var, |
| 55 | + #= x = lda.LD1, |
| 56 | + #= y = lda.LD2), |
| 57 | + #= max.overlaps = getOption("ggrepel.max.overlaps", default = 100)) + |
| 58 | + coord_cartesian(clip = "off") + |
| 59 | + guides(color = guide_legend(override.aes = list(alpha = 1))) + |
| 60 | + labs(x = "LDA 1", |
| 61 | + y = "LDA 2") + |
| 62 | + theme_classic() + |
| 63 | + theme(panel.border = element_rect(colour = 'black', |
| 64 | + fill = "#ffffff00"), |
| 65 | + panel.grid.minor = element_blank(), |
| 66 | + panel.grid.major.x = element_blank(), |
| 67 | + axis.line = element_blank(), |
| 68 | + axis.ticks = element_line(colour = colorspace::darken("#dddddd", 0.1), |
| 69 | + linewidth = 0.3), |
| 70 | + plot.background = element_rect(fill = "white", colour = NA), |
| 71 | + panel.background = element_rect(fill = "white", colour = NA), |
| 72 | + legend.background = element_rect(fill = "white", colour = NA), |
| 73 | + legend.key = element_blank(), |
| 74 | + text = element_text(color = "#5e5e5e"), |
| 75 | + plot.margin = margin(10, 5, 5, 10), |
| 76 | + legend.margin = margin(1, 2, 1, 2) |
| 77 | + ) |
| 78 | + |
| 79 | +ggsave("../figures/lda.png", |
| 80 | + width = 5000, |
| 81 | + height = 4000, |
| 82 | + units = "px", |
| 83 | + dpi = 700) |
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