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laserCtrlGUI.m
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function laserCtrlGUI
% laserCtrlGUI
% GUI to control laser, galvos and video acquisition
%
% Lucas Pinto, Jan 2016
% Princeton Neuroscience Institute
%% INITIALIZE VARIABLES
global lsr obj
lsr = lsrCtrlParams; % get class object with laser parameters
lsr = calculateP_on(lsr); % enforce max prob./location
lsr = getCalValues(lsr); % get calibration parameters and quick-check laser power calibration
[lsr.galvoManualVx,lsr.galvoManualVy] = convertToGalvoVoltage([lsr.ML lsr.AP],'mm'); % galvo voltage
load(sprintf('%s\\grid\\fullGrid.mat',lsr.rootdir),'grid'); % load default grid
lsr.grid = grid;
lsr.gridLabel = 'fullGrid.mat';
lsr.locationSet = num2cell(1:size(lsr.grid,1));
lsr = computeOuputData(lsr); % compute laser/galvo data output
lsr.fn = sprintf('%s_%s',lsr.mouseID,datestr(datetime,'yyyymmdd_HHMMSS')); % default file name
%% start GUI and draw buttons
drawGUIfig; % nested function at the bottom
%% Intialize Galvo and lasers DAQ controls:
if LaserRigParameters.hasDAQ
nidaqComm('init');
end
% update session log
updateConsole('session started')
% if last quick cal was performed longer than 48h ago, do it
set(obj.statusTxt,'foregroundcolor','r'); drawnow()
set(obj.statusTxt,'String','Performing quick power calibration...'); drawnow()
% lsr = quickPowerCal(lsr);
updateConsole(lsr.powerCalcheckMsg)
set(obj.statusTxt,'String','Idle','foregroundcolor',[.3 .3 .3])
end
%%
%==========================================================================
%% CAMERA CALLBACKS
%==========================================================================
% camera on/off
function camON_callback(~,event)
global obj
if get(obj.camON,'Value') == true
% create video input
if ~isfield(obj,'vid'); obj = createVideoObject(obj); end
% go into video data acquisition loop
try
camLoop;
catch
delete(obj.vid)
clear obj.vid
obj = createVideoObject(obj);
camLoop;
end
end
end
% save frame
function grabFrame_callback(~,event)
global obj lsr
if get(obj.grab,'Value') == true
set(obj.camON,'Value',false);
drawnow();
camON_callback([],1);
f1 = figure;
set(f1,'visible','off')
plotGalvoGrid(f1);
uin = questdlg('save as reference image?'); % save?
switch uin
case 'Yes'
thisfn = sprintf('%s%s_refIm',lsr.savepath,lsr.mouseID);
uin2 = questdlg('set reference pixel?'); % prompt to change ref. pxl
if strcmpi(uin2,'Yes')
set(obj.setZero,'Value',1)
setZero_callback([],1)
set(obj.setZero,'Value',0)
end
lsr.refIm = obj.camData;
% save as refIM and also with a date for recordkeeping
frame = obj.camData; refPxl = lsr.refPxl;
saveas(f1,sprintf('%s.fig',thisfn),'fig')
save(thisfn,'frame','refPxl')
imwrite(frame,sprintf('%s.tif',thisfn),'tif')
% reset im registration
lsr.imTform = [];
thisls = dir(sprintf('%s%s_frameGrab*',lsr.savepath,lsr.fn));
if isempty(thisls)
thisfn = sprintf('%s%s_frameGrab',lsr.savepath,lsr.fn);
else
thisfn = sprintf('%s%s_frameGrab-%d',lsr.savepath,lsr.fn,length(thisls));
end
saveas(f1,sprintf('%s.fig',thisfn),'fig')
save(thisfn,'frame','refPxl')
imwrite(frame,sprintf('%s.tif',thisfn),'tif')
case 'No'
thisls = dir(sprintf('%s%s_frameGrab*',lsr.savepath,lsr.fn));
if isempty(thisls)
thisfn = sprintf('%s%s_frameGrab',lsr.savepath,lsr.fn);
else
thisfn = sprintf('%s%s_frameGrab-%d',lsr.savepath,lsr.fn,length(thisls));
end
lsr.currIm = obj.camData;
frame = obj.camData; refPxl = lsr.refPxl;
saveas(f1,sprintf('%s.fig',thisfn),'fig')
save(thisfn,'frame','refPxl')
imwrite(frame,sprintf('%s.tif',thisfn),'tif')
case 'Cancel'
close(f1)
end
set(f1,'visible','on','position',[20 20 obj.vidRes]);
close(f1)
updateConsole(sprintf('image saved to %s',thisfn))
% prompt to register
if strcmpi(uin,'No')
uin3 = questdlg('register to reference image?');
if strcmpi(uin3,'Yes')
set(obj.registerIm,'Value',true)
registerIm_callback([],1);
set(obj.registerIm,'Value',false)
end
end
plotGridAndHeadplate(obj.camfig);
end
end
% use mouse pointer/crosshair to set zero (bregma) on image
function setZero_callback(~,event)
global obj lsr
if get(obj.setZero,'Value') == true
lsr.refPxl = ginput(1);
hold on; plot(lsr.refPxl(1),lsr.refPxl(2),'m+','markersize',10)
lsr = computeOuputData(lsr);
updateConsole('set new reference pixel')
plotGridAndHeadplate(obj.camfig);
end
end
% load stimulation grid
function grid_callback(~,event)
global obj lsr
if get(obj.grid,'Value') == true
thisdir = pwd;
cd([lsr.rootdir '\grid'])
fn = uigetfile('*.mat','select grid file');
cd(thisdir)
% load grid and update parameters
loadgrid([lsr.rootdir '\grid\' fn])
% plot it
plotGridAndHeadplate(obj.camfig);
end
end
function loadgrid(fn)
global lsr obj
load(fn,'grid','P_on')
lsr.P_on = P_on;
lsr.grid = grid;
lsr.gridLabel = fn;
lsr.locationSet = [];
set(obj.pON,'String',num2str(lsr.P_on));
updateConsole(sprintf('loaded %s',fn))
if iscell(lsr.grid) % grid indicates simulatenous regions (each cell has the coordinates for those)
for ii = 1:length(grid)
lsr.locationSet{ii} = 1:size(lsr.grid{ii},1);
end
else
lsr.locationSet = num2cell(1:size(lsr.grid,1));
end
if ~strcmpi(lsr.gridLabel,'cfos.mat')
lsr = computeOuputData(lsr);
end
end
% set stimulation grid with cursor
function setgrid_callback(~,event)
global obj lsr
if get(obj.setgrid,'Value') == true
axes(obj.camfig); % focus
cla
imagesc(obj.camData); colormap gray; axis image;
set(gca,'XDir','reverse','xtick',[],'ytick',[]);
stopSelection = 0;
grid = [];
while ~stopSelection
pxlin = round(ginput(1));
x = (-pxlin(1) + lsr.refPxl(1))/lsr.pxlPerMM;
y = (-pxlin(2) + lsr.refPxl(2))/lsr.pxlPerMM;
grid(end+1,:) = [x y];
uin = questdlg('Select more locations?');
if strcmpi(uin,'Yes')
stopSelection = 0;
elseif strcmpi(uin,'No')
stopSelection = 1;
end
end
% save new grid
thisdir = pwd;
cd([lsr.rootdir '\grid'])
fn = uiputfile('*.mat','save new grid as');
save(fn,'grid')
cd(thisdir)
updateConsole(sprintf('new manual grid saved to %s',fn))
% update output data etc
lsr.grid = grid;
lsr.locationSet = num2cell(1:size(lsr.grid,1));
lsr = computeOuputData(lsr);
% update lsr on prob. according to grid size if necessary
prevPon = lsr.P_on;
if numel(lsr.locationSet) > 1/lsr.maxPonPerLoc && prevPon <= lsr.maxPonPerLoc
lsr.P_on = 0.8;
end
lsr = calculateP_on(lsr);
if prevPon > lsr.P_on
set(obj.pON,'String',num2str(lsr.P_on));
updateConsole(sprintf('laser on prob. capped at %1.2f',lsr.P_on))
elseif prevPon < lsr.P_on
set(obj.pON,'String',num2str(lsr.P_on));
updateConsole(sprintf('laser on prob. automatically increased to %1.2f',lsr.P_on))
end
P_on = lsr.P_on;
save(fn,'P_on','-append')
% plot it
plotGridAndHeadplate(obj.camfig);
end
end
% register iamge
function registerIm_callback(~,event)
global obj lsr
if get(obj.registerIm,'Value') == true
if isempty(lsr.currIm)
thisdir = pwd;
cd(lsr.savepath)
thisfn = uigetfile('*.tif','select image');
frame = imread(thisfn);
cd(thisdir)
lsr.currIm = obj.camData;
end
set(obj.statusTxt,'String','performing Im regsitration...')
drawnow()
[regMsg,lsr.okFlag] = registerImage(lsr.refIm,lsr.currIm,false);
wd = warndlg(regMsg,'Registration output');
set(obj.statusTxt,'String','Idle')
updateConsole('image registered')
end
end
% load stimulation grid
function drawHeadplate_callback(~,event)
global obj lsr
if get(obj.drawHeadplate,'Value') == true
% manually draw headplate
drawHeadplate(lsr.savepath,lsr.mouseID)
% plot it
plotGridAndHeadplate(obj.camfig);
end
end
% Green LED ON/OFF callback
function ledGreen_callback(~,event)
global obj
obj.LEDdataout(LaserRigParameters.LEDIdxGreen) = get(obj.LEDgreen,'Value');
if LaserRigParameters.hasDAQ
nidaqDOwrite('writeDO',obj.LEDdataout)
end
end
% IR LED ON/OFF callback
function ledIR_callback(~,event)
global obj
obj.LEDdataout(LaserRigParameters.LEDIdxIR) = get(obj.LEDir,'Value');
if LaserRigParameters.hasDAQ
nidaqDOwrite('writeDO',obj.LEDdataout)
end
end
%%
%==========================================================================
%% GENERAL CTRL CALLBACKS
%==========================================================================
% set directory for file saving
function cd_callback(~,event)
global obj lsr
if event == true || get(obj.sdir,'Value') == true
lsr.savepath = uigetdir(lsr.rootdir,'Pick a directory');
refreshFn_callback([],1);
end
end
% select mouse
function subjList_callback(~,event)
global obj lsr
if strcmpi(obj.subjList{get(obj.subjListDrop,'Value')},'add new')
newmouse = inputdlg({'mouse ID:'});
obj.animalListObj = obj.animalListObj.addToList(newmouse);
lsr.mouseID = newmouse{1};
refreshFn_callback([],1);
set(obj.subjListDrop,'String',obj.animalListObj.mouseList)
set(obj.subjListDrop,'Value',length(obj.animalListObj.mouseList)-1)
else
midx = get(obj.subjListDrop,'Value');
lsr.mouseID = obj.subjList{midx};
refreshFn_callback([],1);
end
% create directory for animal if necessary
if isempty(dir(sprintf('%s%s',lsr.savepathroot,lsr.mouseID)))
mkdir(sprintf('%s%s',lsr.savepathroot,lsr.mouseID));
end
% change savepath
lsr.savepath = [lsr.savepathroot lsr.mouseID '\'];
% load reference image
if ~isempty(dir(sprintf('%s%s_refIm.mat',lsr.savepath,lsr.mouseID)))
load(sprintf('%s%s_refIm',lsr.savepath,lsr.mouseID),'frame','refPxl')
lsr.refIm = frame;
lsr.refPxl = refPxl;
else
thish = warndlg('reference image not found');
end
% load headplate outline
if ~isempty(dir(sprintf('%s%s_headplate.mat',lsr.savepath,lsr.mouseID)))
load(sprintf('%s%s_headplate.mat',lsr.savepath,lsr.mouseID),'headplateContour')
lsr.headplateOutline = headplateContour;
else
thish = warndlg('headplate outline not found');
end
% retrieve and set default parameters for this animal
power = animalList.powerList{midx};
grid = animalList.gridList{midx};
varpower = animalList.varPower(midx);
epoch = animalList.epochList{midx};
epochVal = find(strcmpi(lsr.epochList,epoch));
set(obj.power,'String', num2str(power)); laserpower([],true);
set(obj.epoch,'Value' , epochVal); epoch_callback([],true)
set(obj.varypower,'Value',varpower); varypower_callback([],true);
loadgrid([lsr.rootdir '\grid\' grid])
if isfield(obj,'camData'); plotGridAndHeadplate(obj.camfig); end
runOnLsr = animalList.runOnLsr(midx);
if runOnLsr
thish = warndlg('Run this mouse on laser');
else
thish = warndlg('Just training for this mouse');
end
end
% file name
function fn_callback(~,event)
global obj lsr
lsr.fn = get(obj.fnenter,'String');
end
% refresh file name
function refreshFn_callback(~,event)
global obj lsr
lsr.fn = sprintf('%s_%s',lsr.mouseID,datestr(datetime,'yyyymmdd_HHMMSS'));
set(obj.fnenter,'String',lsr.fn)
end
% reset
function reset_callback(~,event)
global obj lsr
if get(obj.resetgui,'Value') == true
if isempty(lsr.console_fn) || isempty(dir(lsr.console_fn))
usrin = questdlg('save session log?');
if strcmpi(usrin,'Yes')
saveConsole([],1);
end
end
% close daq communication
if LaserRigParameters.hasDAQ == true
nidaqComm('end');
end
close(obj.fig); clear
laserCtrlGUI
end
end
% quit GUI
function quitgui_callback(~,event)
global obj lsr
if get(obj.quitgui,'Value') == true
if isempty(lsr.console_fn) || isempty(dir(lsr.console_fn))
usrin = questdlg('save session log?');
if strcmpi(usrin,'Yes')
saveConsole([],1);
end
end
% close daq communication
if LaserRigParameters.hasDAQ == true
nidaqComm('end');
end
close(obj.fig); clear
end
end
%%
% =========================================================================
%% LASER PARAMETER CALLBACKS
%==========================================================================
% set source (manual or external trigger)
function src_callback(~,event)
global obj lsr
if strcmpi(get(obj.src.SelectedObject,'string'),'manual')
lsr.manualTrigger = true;
else
lsr.manualTrigger = false;
end
end
% set pulse frequency
function laserfreq(~,event)
global obj lsr
lsr.freq = str2double(get(obj.freq,'String'));
lsr = computeOuputData(lsr);
updateConsole(sprintf('laser frequency changed to %s Hz',get(obj.freq,'String')))
end
% set pulse duration
function laserdur(~,event)
global obj lsr
lsr.dur = str2double(get(obj.dur,'String'));
end
% set pulse duty cycle
function laserduty(~,event)
global obj lsr
lsr.dutyCycle = str2double(get(obj.dutyCycle,'String'));
lsr = computeOuputData(lsr);
updateConsole(sprintf('laser duty cycle changed to %s',get(obj.dutyCycle,'String')))
end
% set laser power with input box
function laserpower(~,event)
global obj lsr
% first make sure it doesn't exceed max voltage, then update
pp = str2double(get(obj.power,'String')); %#ok<*ST2NM>
if pp <= lsr.maxP
lsr.power = pp;
else
lsr.power = lsr.maxP;
warndlg('Power exceeds allowed max, set to max')
end
lsr.Vlsr = (lsr.power-lsr.b_power)/lsr.a_power;
lsr = computeOuputData(lsr);
updateConsole(sprintf('laser power changed to %1.1f mW',lsr.power))
end
% set ramp dpwn duartion with input box
function rampdowndur(~,event)
global obj lsr
lsr.rampDownDur = str2double(get(obj.rampdown,'String'));
updateConsole(sprintf('ramp down duration changed to %s s',get(obj.rampdown,'String')))
end
% set laser ON trial probability with input box
function pON_callback(~,event)
global obj lsr
lsr.P_on = str2double(get(obj.pON,'String'));
lsr = calculateP_on(lsr);
set(obj.pON,'String',num2str(lsr.P_on));
updateConsole(sprintf('laser on prob. changed to %1.2f',lsr.P_on))
end
% select laser ON trial epoch with drop down menu
function epoch_callback(~,event)
global obj lsr
lsr.epoch = lsr.epochList{get(obj.epoch,'Value')};
updateConsole(sprintf('trial epoch changed to %s',lsr.epoch))
end
% select ramp down method with drop down menu
function rampmethod_callback(~,event)
global obj lsr
lsr.rampDownMode = lsr.rampDownList{get(obj.rampmethod,'Value')};
updateConsole(sprintf('ramp down mode changed to %s',lsr.rampDownMode))
end
% select laser ON trial epoch with drop down menu
function trialdraw_callback(~,event)
global obj lsr
lsr.drawMode = lsr.drawModeList{get(obj.trialdraw,'Value')};
updateConsole(sprintf('trial drawing method changed to %s',lsr.drawMode))
end
% select laser ON trial epoch with drop down menu
function varypower_callback(~,event)
global obj lsr
lsr.varyPower = get(obj.varypower,'Value');
if lsr.varyPower
updateConsole(sprintf('laser power will be randomly varied'))
else
updateConsole(sprintf('constant laser power'))
end
end
% set AP position with text input
function posX_callback(~,event)
global obj lsr
lsr.ML = str2double(get(obj.posX,'String'));
[lsr.galvoManualVx,lsr.galvoManualVy] = convertToGalvoVoltage([lsr.ML lsr.AP],'mm');
lsr = computeOuputData(lsr);
updateConsole('galvo position manually updated')
end
% set ML position with text input
function posY_callback(~,event)
global obj lsr
lsr.AP = str2double(get(obj.posY,'String'));
[lsr.galvoManualVx,lsr.galvoManualVy] = convertToGalvoVoltage([lsr.ML lsr.AP],'mm');
lsr = computeOuputData(lsr);
updateConsole('galvo position manually updated')
end
% select galvo location with cursor
function manualGalvo_callback(~,event)
global obj lsr
if get(obj.manualSelect,'Value') == true
galvoClickControl;
updateConsole('galvo position manually updated')
end
end
% execute new galvo position
function goto_callback(~,event)
global obj lsr
if get(obj.goToPos,'Value') == true && lsr.manualTrigger == true
dataout = zeros(1,4);
dataout(LaserRigParameters.galvoCh(1)) = lsr.dataout_manual.galvoXvec(1);
dataout(LaserRigParameters.galvoCh(2)) = lsr.dataout_manual.galvoYvec(1);
dataout(LaserRigParameters.lsrWaveCh) = lsr.dataout_manual.lsrVec(1);
dataout(LaserRigParameters.lsrSwitchCh) = 5;
nidaqAOPulse('aoPulse',dataout);
% update status
set(obj.statusTxt,'foregroundColor','b')
set(obj.statusTxt,'String','constant pulse')
elseif get(obj.goToPos,'Value') == true && lsr.manualTrigger == false
warndlg('Please enable manual trigger first')
else
nidaqAOPulse('aoPulse',[0 0 0 0]);
% update status
set(obj.statusTxt,'foregroundColor',[.3 .3 .3])
set(obj.statusTxt,'String','Idle')
end
end
% laser on / off
function pulse_callback(~,event)
global obj lsr
if get(obj.pulse,'Value') == true && lsr.manualTrigger == true
if get(obj.camON,'Value') == true % can't run loop and read cam at the same time
else
laserLoop;
end
elseif get(obj.pulse,'Value') == true && lsr.manualTrigger == false
warndlg('Please enable manual trigger first')
end
end
% start behavioral experiment
function ready_callback(~,event)
global obj lsr
if get(obj.ready,'Value') == true
if lsr.manualTrigger == true
warndlg('Please enable external trigger first')
else
if lsr.okFlag
laserLoop;
lsr.okFlag = false;
else
warndlg('Aligment is off. Please fix before proceding')
end
end
end
end
%%
% =========================================================================
%% EXPERIMENT CONTROL CALLBACKS
%==========================================================================
function deleteInstructions(src,event)
global obj
if sum(get(obj.logTxt,'foregroundcolor')) > 0
set(obj.logTxt,'String','','foregroundcolor',[0 0 0]);
end
end
% save notes to text file
function saveNotes(~,event)
global obj lsr
% time stamp for note
temp = datetime;
calDate = datestr(temp,'HH:MM:SS');
lsr.note_fn=[lsr.savepath '\experNotes' lsr.fn '.txt'];
% creating or appending?
if isempty(dir(lsr.note_fn))
obj.noteAppended = 0;
else
obj.noteAppended = 1;
end
% retrieve note and save to .txt file
set(obj.logTxt,'Selected','off');
thisstr = get(obj.logTxt,'String');
while isempty(thisstr)
thisstr = get(obj.logTxt,'String');
end
thisstr = textwrap({thisstr},30);
fid = fopen(lsr.note_fn,'a+');
fprintf(fid,'\nnote at %sh\n',calDate);
for ii = 1:length(thisstr)
fprintf(fid,'%s\r\n',thisstr{ii});
end
fprintf(fid,'\r\n');
fclose(fid);
% reset box and output action
set(obj.logTxt,'String','Enter notes here','foregroundcolor',[.7 .7 .7]);
if obj.noteAppended
updateConsole(sprintf('appended to experNotes%s',lsr.fn))
else
updateConsole(sprintf('created experNotes%s',lsr.fn))
end
end
% save console to text file
function saveConsole(~,event)
global obj lsr
lsr.console_fn=[lsr.savepath '\sessionLog' lsr.fn '.txt'];
% retrieve note and save to .txt file
thisstr = get(obj.outputTxt,'String');
fid = fopen(lsr.console_fn,'a+');
for ii = 1:length(thisstr)
fprintf(fid,'\r\n%s',thisstr{ii});
end
fprintf(fid,'\r\n');
fclose(fid);
% reset console
set(obj.outputTxt,'String', ...
{'------------------------------------------'; ...
[' ' datestr(datetime)] ; ...
'------------------------------------------'; ...
'' });
end
%%
% =========================================================================
%% CALIBRATION CALLBACKS
%==========================================================================
% power
function powercal_callback(~,event)
global obj lsr
if get(obj.powercal,'Value') == true
powerCal;
figure(obj.fig)
updateConsole('power calibration')
lsr = getCalValues(lsr);
lsr = computeOuputData(lsr);
end
end
% galvos
function galvocal_callback(~,event)
global obj lsr
if get(obj.galvocal,'Value') == true
galvoCal;
figure(obj.fig)
updateConsole('galvo calibration')
lsr = getCalValues(lsr);
lsr = computeOuputData(lsr);
end
end
% sweep galvos
function galvosweep_callback(~,event)
global obj lsr
if get(obj.galvosweep,'Value') == true
dataout = zeros(1,4);
dataout(LaserRigParameters.lsrSwitchCh) = 5;
dataout(LaserRigParameters.lsrWaveCh) = lsr.Vlsr;
GridSizeX = 11;
GridSizeY = 11;
VxMin = -1.5; VxMax = 1.5;
VyMin = -1.0; VyMax = 1.0;
for i=1:GridSizeX
for j=1:GridSizeY
Vx = (VxMax-VxMin)*(i-1)/(GridSizeX-1) + VxMin;
Vy = (VyMax-VyMin)*(j-1)/(GridSizeY-1) + VyMin;
dataout(LaserRigParameters.galvoCh(1)) = Vx;
dataout(LaserRigParameters.galvoCh(2)) = Vy;
nidaqAOPulse('aoPulse',dataout);
delay(.2);
end
end
dataout = zeros(1,4);
nidaqAOPulse('aoPulse',dataout);
end
end
%%
% =========================================================================
%% PRESET CALLBACKS
%==========================================================================
% example preset used in ephys confirmation experiments (Pinto et al 2019)
function ephyspreset_callback(~,event)
global obj lsr
if get(obj.ephyspreset,'Value') == true
load([lsr.rootdir '\grid\ephys.mat'],'grid','locDur','cycleDur','powers','ntrials','rampDown')
updateConsole('loaded ephys.mat')
lsr.grid = grid;
lsr.gridLabel = 'ephys.mat';
lsr.preSetOn = true;
lsr.presetLocDur = locDur; % duration per setim spot in sec
lsr.presetCycleDur = cycleDur; % total cycle duration in sec
lsr.presetNTrials = ntrials; % expt duration in min
lsr.presetMaxDurMin = inf;
lsr.presetPowers = powers;
lsr.presetRampDown = rampDown;
lsr.ephys = true;
for ii = 1:length(powers)
lsr.Vlsr_preset(ii) = (powers(ii)-lsr.b_power)/lsr.a_power;
end
lsr.Vlsr_preset(lsr.Vlsr_preset>5) = 5;
lsr.locationSet = [];
if iscell(lsr.grid) % grid indicates simulatenous regions (each cell has the coordinates for those)
for ii = 1:length(grid)
lsr.locationSet{ii} = 1:size(lsr.grid{ii},1);
end
else
lsr.locationSet = num2cell(1:size(lsr.grid,1));
end
lsr = computeOuputDataPreSetEphys(lsr);
plotGridAndHeadplate(obj.camfig);
laserLoop;
end
end
%%
% =========================================================================
%% DRAW GUI OBJECT
function drawGUIfig
global obj lsr
obj.animalListObj = animalList;
obj.subjList = obj.animalListObj.mouseList;
obj.subjList{end+1} = 'add new';
obj.consoleInitString = {'------------------------------------------'; ...
[' ' datestr(datetime)] ; ...
'------------------------------------------'; ...
'' };
% create video object
% imaqreset;
% obj.vid = videoinput('pointgrey', 1, 'F7_Mono16_1920x1200_Mode7');
% create GUI figure
ss = get(groot,'screensize');
ss = ss(3:4);
obj.fig = figure ('Name', 'Laser Control', ...
'NumberTitle', 'off', ...
'Position', round([ss(1)*.1 ss(2)*.1 ss(1)*.8 ss(2)*.8]));
% -------------------------------------------------------------------------
%% general controls
% -------------------------------------------------------------------------
obj.subjtxt = uicontrol (obj.fig, ...
'Style', 'text', ...
'String', 'Mouse ID:', ...
'Units', 'normalized', ...
'Position', [.028 .052 .07 .04],...
'horizontalAlignment', 'left', ...
'fontsize', 13, ...
'fontweight', 'bold');
obj.subjListDrop = uicontrol (obj.fig, ...
'Style', 'popupmenu', ...
'String', obj.subjList, ...
'Units', 'normalized', ...
'Position', [.028 .02 .08 .038],...
'horizontalAlignment', 'left', ...
'fontsize', 13, ...
'Callback', @subjList_callback);
obj.fntxt = uicontrol (obj.fig, ...
'Style', 'text', ...
'String', 'File name:', ...
'Units', 'normalized', ...
'Position', [.14 .052 .07 .04], ...
'horizontalAlignment', 'left', ...
'fontsize', 13, ...
'fontweight', 'bold');
obj.fnenter = uicontrol (obj.fig, ...
'Style', 'edit', ...
'String', lsr.fn, ...
'Units', 'normalized', ...
'Position', [.14 .022 .15 .038],...
'horizontalAlignment', 'left', ...
'fontsize', 13, ...
'Callback', @fn_callback);
obj.refreshFn = uicontrol (obj.fig, ...
'String', 'refresh', ...
'Style', 'pushbutton', ...
'Units', 'normalized', ...
'Position', [.291 .0225 .05 .038],...
'Callback', @refreshFn_callback,...
'fontsize', 12);
obj.sdir = uicontrol (obj.fig, ...
'String', 'set dir', ...
'Style', 'pushbutton', ...
'Units', 'normalized', ...
'Position', [.40 .02 .07 .05], ...
'Callback', @cd_callback, ...
'fontsize', 13, ...
'fontweight', 'bold');
obj.resetgui = uicontrol (obj.fig, ...
'String', 'RESET', ...
'Style', 'pushbutton', ...
'Units', 'normalized', ...
'Position', [.47 .02 .07 .05], ...
'Callback', @reset_callback, ...
'fontsize', 13, ...
'foregroundColor', [1 .6 .1], ...
'fontweight', 'bold');
obj.quitgui = uicontrol (obj.fig, ...
'String', 'QUIT', ...
'Style', 'pushbutton', ...
'Units', 'normalized', ...
'Position', [.54 .02 .07 .05], ...
'foregroundColor', [1 0 0], ...
'Callback', @quitgui_callback, ...
'fontsize', 13, ...
'fontweight', 'bold');
% -------------------------------------------------------------------------
%% camera feedback panel
% -------------------------------------------------------------------------
obj.vidpan = uipanel ('Parent', obj.fig, ...
'Title', 'Camera', ...
'Units', 'normalized', ...
'Position', [.03 .1 .58 .86], ...
'fontsize', 14, ...
'fontweight', 'bold');
obj.camfig = axes ('units', 'normalized', ...
'position', [.02 .15 .96 .8], ...
'parent', obj.vidpan, ...
'visible', 'off', ...
'xtick', [], ...
'ytick', []);
obj.camON = uicontrol (obj.vidpan, ...
'String', 'cam ON', ...
'Style', 'togglebutton', ...
'Units', 'normalized', ...
'Position', [.01 .02 .10 .07], ...
'Callback', @camON_callback, ...
'fontsize', 13);
obj.grab = uicontrol (obj.vidpan, ...
'String', 'grab frame', ...
'Style', 'pushbutton', ...
'Units', 'normalized', ...
'Position', [.115 .02 .10 .07], ...
'Callback', @grabFrame_callback,...
'fontsize', 13);
obj.registerIm = uicontrol (obj.vidpan, ...
'String', 'register', ...
'Style', 'pushbutton', ...
'Units', 'normalized', ...
'Position', [.22 .02 .10 .07], ...
'Callback', @registerIm_callback,...
'fontsize', 13);
obj.setZero = uicontrol (obj.vidpan, ...
'String', 'set zero', ...
'Style', 'pushbutton', ...
'Units', 'normalized', ...
'Position', [.325 .02 .10 .07], ...
'Callback', @setZero_callback, ...
'fontsize', 13);
obj.grid = uicontrol (obj.vidpan, ...
'String', 'load grid', ...
'Style', 'pushbutton', ...
'Units', 'normalized', ...
'Position', [.43 .02 .10 .07], ...
'Callback', @grid_callback, ...
'fontsize', 13);
obj.setgrid = uicontrol (obj.vidpan, ...
'String', 'set grid', ...
'Style', 'pushbutton', ...
'Units', 'normalized', ...
'Position', [.535 .02 .10 .07], ...
'Callback', @setgrid_callback, ...
'fontsize', 13);
obj.drawHeadplate = uicontrol (obj.vidpan, ...
'String', 'draw plate' , ...
'Style', 'pushbutton', ...
'Units', 'normalized', ...
'Position', [.64 .02 .10 .07], ...
'Callback', @drawHeadplate_callback, ...