-
Notifications
You must be signed in to change notification settings - Fork 0
/
Copy pathSeq.java
1392 lines (1228 loc) · 38.3 KB
/
Seq.java
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
689
690
691
692
693
694
695
696
697
698
699
700
701
702
703
704
705
706
707
708
709
710
711
712
713
714
715
716
717
718
719
720
721
722
723
724
725
726
727
728
729
730
731
732
733
734
735
736
737
738
739
740
741
742
743
744
745
746
747
748
749
750
751
752
753
754
755
756
757
758
759
760
761
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
778
779
780
781
782
783
784
785
786
787
788
789
790
791
792
793
794
795
796
797
798
799
800
801
802
803
804
805
806
807
808
809
810
811
812
813
814
815
816
817
818
819
820
821
822
823
824
825
826
827
828
829
830
831
832
833
834
835
836
837
838
839
840
841
842
843
844
845
846
847
848
849
850
851
852
853
854
855
856
857
858
859
860
861
862
863
864
865
866
867
868
869
870
871
872
873
874
875
876
877
878
879
880
881
882
883
884
885
886
887
888
889
890
891
892
893
894
895
896
897
898
899
900
901
902
903
904
905
906
907
908
909
910
911
912
913
914
915
916
917
918
919
920
921
922
923
924
925
926
927
928
929
930
931
932
933
934
935
936
937
938
939
940
941
942
943
944
945
946
947
948
949
950
951
952
953
954
955
956
957
958
959
960
961
962
963
964
965
966
967
968
969
970
971
972
973
974
975
976
977
978
979
980
981
982
983
984
985
986
987
988
989
990
991
992
993
994
995
996
997
998
999
1000
import java.util.*;
/**
* Seq is the base class for holding dna sequence datas.
*
* @author Heewook Lee
* @date Jan 25 2007
* @updataed July 03, 2007
* @ver 1.1
*/
public class Seq{
private StringBuffer seqBuffer; // global variable for holding sequence data in StringBuffer Obj
private String seqName; // golbal variable for holding sequence name in Sting Obj
/*
* Constructor
*
* @param fastaName name of the fasta seq
* @param seq sequence in String obj
*
*/
public Seq(String fastaName, String seq){
seqName = fastaName;
seqBuffer = new StringBuffer(seq);
}
public int[] countDinucleotide(char firstBase){
int[] count = new int[4];//ACGT
for(int i=0;i<(seqBuffer.length()-1);i++){
if(seqBuffer.charAt(i)==firstBase){
char nb = seqBuffer.charAt(i+1);
if(nb=='A')
count[0]++;
else if(nb == 'C')
count[1]++;
else if(nb =='G')
count[2]++;
else if(nb =='T')
count[3]++;
}
}
return count;
}
/*
* A: 0 C: 1 G: 2 T: 3
*
*/
public int[] countAllDinucleotide(){
int[] dimerCount = new int[16];
for(int i=0; i<(seqBuffer.length()-1); i++){
dimerCount[this.baseToNumber(seqBuffer.charAt(i)) * 4 + this.baseToNumber(seqBuffer.charAt(i+1))]++;
}
dimerCount[this.baseToNumber(seqBuffer.charAt(seqBuffer.length()-1)) * 4 + this.baseToNumber(seqBuffer.charAt(0))]++;
return dimerCount;
}
public int[] countAllTrinucleotide(){
int[] trimerCount = new int[64];
for(int i=0; i<(seqBuffer.length()-2); i++){
trimerCount[this.baseToNumber(seqBuffer.charAt(i)) * 16
+ this.baseToNumber(seqBuffer.charAt(i+1)) * 4
+ this.baseToNumber(seqBuffer.charAt(i+2)) ]++;
}
trimerCount[this.baseToNumber(seqBuffer.charAt(seqBuffer.length()-2)) * 16
+ this.baseToNumber(seqBuffer.charAt(seqBuffer.length()-1)) * 4
+ this.baseToNumber(seqBuffer.charAt(0)) ]++;
trimerCount[this.baseToNumber(seqBuffer.charAt(seqBuffer.length()-1)) * 16
+ this.baseToNumber(seqBuffer.charAt(0)) * 4
+ this.baseToNumber(seqBuffer.charAt(1)) ]++;
return trimerCount;
}
public int baseToNumber(char base){
if(base == 'A' || base == 'a')
return 0;
else if(base == 'C' || base == 'c')
return 1;
else if(base == 'G' || base == 'g')
return 2;
else if(base == 'T' || base == 't')
return 3;
else
return -1;
}
/*
* Constructor
*
* @param fastaName name of the fasta seq
* @param seq sequence in String obj
*
*/
public Seq(String fastaName, StringBuffer seq){
seqName = fastaName;
seqBuffer = seq;
}
/*
* Constructor
*
* @param fastaName name of the fasta seq
*
*/
public Seq(String fastaName){
seqName = fastaName;
seqBuffer = new StringBuffer();
}
public Seq(){
}
public Gene toGene(){
return new Gene(seqName, 1, seqBuffer.length(), true, seqName);
}
/*
* getSeqName() method simple returns the seqName
*
* @return seqName String obj seqName is returned
*/
public String getSeqName(){
return seqName;
}
/*
* getSeq() method simple returns the sequence buffer
*
* @return seqBuffer StringBuffer obj sequence is returned
*/
public StringBuffer getSeq(){
return new StringBuffer(this.seqBuffer.toString());
}
public int getSeqLength(){
return this.seqBuffer.length();
}
public Seq deepCopy(){
Seq newer = new Seq();
newer.seqBuffer = this.getSeq();
newer.seqName = this.seqName;
return newer;
}
public Seq getRevSeq(){
return new Seq(this.seqName, new StringBuffer(this.revSeqInternal().toString()));
}
/*
* formatSeq() method returns the sequence in fasta format without the seq name tag
* each line containts 50 neucleotide bases.
*
* @return outBuffer StringBuffer obj sequnce is returned with newline character every 50 bases
*/
public StringBuffer formatSeq(){
StringBuffer outBuffer = new StringBuffer();
int lowInd = 0;
for(int i=0; i<seqBuffer.length();i++){
if((i % 50) == 49){
outBuffer.append(seqBuffer.substring(lowInd,i+1));
outBuffer.append("\n");
lowInd += 50;
}
}
if( (seqBuffer.length()%50) != 0){
outBuffer.append(seqBuffer.substring(lowInd));
outBuffer.append("\n");
}
return outBuffer;
}
public ArrayList<IntegerCounter> countRuns(){
//System.err.println("TOTAL LEN : " + this.seqBuffer.length());
ArrayList<IntegerCounter> runCountArr = new ArrayList<IntegerCounter>();
int curRunLen = 0;
char curRunChar = '0';
for(int i=0;i<this.seqBuffer.length();i++){
char curChar = Character.toUpperCase(seqBuffer.charAt(i));
if(curChar == curRunChar)//if it's a currentRun
curRunLen++;
else{/* need to update now*/
if(curRunLen > 0){
if(runCountArr.size() < curRunLen)
updateRunArr(runCountArr,curRunLen);
runCountArr.get(curRunLen-1).add1(curRunChar);
}
//if(curRunLen == 10)
// System.err.println(i + "\t" + curRunChar);
curRunLen = 1;
curRunChar = curChar;
}
}
if(curRunLen > 0 )
runCountArr.get(curRunLen-1).add1(curRunChar);
return runCountArr;
}
public void updateRunLengthArr(ArrayList<RunLengthGeneCounter> arr, int runLen ){
int l = runLen + 1;
while(arr.size() < runLen){
arr.add(new RunLengthGeneCounter(l));
l++;
}
}
private void initRunLengthArr(ArrayList<RunLengthGeneCounter> arr){
for(int i=0; i<10; i++){
arr.add(new RunLengthGeneCounter(i+1));
}
}
public ArrayList<RunLengthGeneCounter> getRuns(ArrayList<Gene> geneList){
ArrayList<RunLengthGeneCounter> runLengthCountArr = new ArrayList<RunLengthGeneCounter>();
this.initRunLengthArr(runLengthCountArr);
int curRunLen = 0;
char curRunChar = '0';
for(int i=0;i<this.seqBuffer.length();i++){
char curChar = Character.toUpperCase(seqBuffer.charAt(i));
if(curChar == curRunChar)//if it's a currentRun
curRunLen++;
else{/* need to update now*/
if(runLengthCountArr.size() < curRunLen)
this.updateRunLengthArr(runLengthCountArr, curRunLen);
if(curRunLen > 3)
runLengthCountArr.get(curRunLen-1).addPosition( ( i - curRunLen + 1), curRunChar, geneList);
curRunLen = 1;
curRunChar = curChar;
}
}
if(runLengthCountArr.size() < curRunLen)
this.updateRunLengthArr(runLengthCountArr, curRunLen);
if(curRunLen > 1 )
runLengthCountArr.get(curRunLen-1).addPosition( ( this.seqBuffer.length() - curRunLen + 1), this.seqBuffer.charAt(this.seqBuffer.length()-1) , geneList);
return runLengthCountArr;
}
/* ACGT, row<ACGT>, col<ACGT>
*
* This method outputs a 3 dimensional array: basically 4 2-dimensional array
* where each array is 4 by 4 <ACGT> vs <ACGT> where row is the 5' neighbor base and col is the 3' neighbor base
*
*/
public int[][][] getNeighboringBaseCountMatrix(){
int[][][] nbcMatrix = new int[4][4][4];
char startBase = Character.toUpperCase(this.seqBuffer.charAt(0));
char endBase = Character.toUpperCase(this.seqBuffer.charAt(this.seqBuffer.length()-1));
char prevBase = endBase;
char curBase = startBase;//Character.toUpperCase(this.seqBuffer.charAt(0));
char nextBase = '0';
for(int i=1;i<this.seqBuffer.length();i++){
nextBase = Character.toUpperCase(this.seqBuffer.charAt(i));
nbcMatrix[this.baseToIndex(curBase)][this.baseToIndex(prevBase)][this.baseToIndex(nextBase)]++;
prevBase = curBase;
curBase = nextBase;
}
nextBase = startBase;
nbcMatrix[this.baseToIndex(curBase)][this.baseToIndex(prevBase)][this.baseToIndex(nextBase)]++;
return nbcMatrix;
}
private int baseToIndex(char b){
if(b == 'A')
return 0;
else if(b == 'C')
return 1;
else if(b == 'G')
return 2;
else if(b == 'T')
return 3;
else
return -1;
}
private void updateRunArr(ArrayList<IntegerCounter> arr, int curRunLen){
while(arr.size() < curRunLen){
arr.add(new IntegerCounter());
}
}
public ArrayList<Integer>[] getMotifLoci(String motif, boolean bothStrands){
int fc = 0;
int rc = 0;
ArrayList<Integer>[] lociArr = null;
if(bothStrands){
lociArr = new ArrayList[2];
lociArr[1] = new ArrayList<Integer>(); /* for rev strand */
}else
lociArr = new ArrayList[1];
lociArr[0] = new ArrayList<Integer>(); /* for fwd strand */
int i = 0;
while(i<this.seqBuffer.length()){
int tmp =this.seqBuffer.indexOf(motif, i);
if(tmp < 0)
i = this.seqBuffer.length();
else{
lociArr[0].add(new Integer(tmp+1));
fc++;
i = tmp + 1;
}
}
if(bothStrands){
StringBuffer rev = revSeqInternal();
i = 0;
while(i<rev.length()){
int tmp = rev.indexOf(motif, i);
if(tmp < 0)
i = this.seqBuffer.length();
else{
lociArr[1].add(new Integer(rev.length() - tmp));
rc++;
i = tmp + 1;
}
}
}
System.err.println("Fwd:\t" + fc);
System.err.println("Rev:\t" + rc);
return lociArr;
}
//thi is to support extended nucleotide bases (IUPAC codes)
public ArrayList<Integer>[] getMotifLociExtendedAlphabets(String motif, boolean bothStrands){
//System.out.println("SEQ:\t" + this.seqBuffer + "\tMOTIF:\t" + motif);
int fc = 0;
int rc = 0;
ArrayList<Integer>[] lociArr = null;
if(bothStrands){
lociArr = new ArrayList[2];
lociArr[1] = new ArrayList<Integer>(); /* for rev strand */
}else
lociArr = new ArrayList[1];
lociArr[0] = new ArrayList<Integer>(); /* for fwd strand */
int i = 0;
while(i<this.seqBuffer.length()){
//System.err.println("fromIndex:\t" + i);
int tmp =this.indexOfNextMotif(motif, i);
if(tmp < 0){
//System.err.println("HIT\t" + i);
i = this.seqBuffer.length();
}else{
lociArr[0].add(new Integer(tmp+1));
fc++;
i = tmp + 1;
}
}
if(bothStrands){
//StringBuffer rev = revSeqInternal();
Seq rev = this.getRevSeq();
i = 0;
while(i<rev.getSeqLength()){
int tmp = rev.indexOfNextMotif(motif, i);
if(tmp < 0){
//System.err.println("REVHIT\t" + i);
i = this.seqBuffer.length();
}else{
lociArr[1].add(new Integer(rev.getSeqLength() - tmp));
rc++;
i = tmp + 1;
}
}
}
System.err.println("Fwd:\t" + fc);
System.err.println("Rev:\t" + rc);
return lociArr;
}
private int indexOfNextMotif(String motif, int fromIndex){
for(int i = fromIndex; i<(this.seqBuffer.length()-motif.length()+1); i++){
if(isMotifMatchAt(motif, i))
return i;
}
return -1;//if not match is found.
}
private boolean isMotifMatchAt(String motif, int index){
int i;
int j = 0;
for(i=index; i<this.seqBuffer.length() && j<motif.length();i++ ){
if(!this.matchBaseAt(i,motif.charAt(j)))
return false;
j++;
}
//System.err.println("Do we ever match Motif??");
return true;
}
//0-base index
private boolean matchBaseAt(int position, char base){
if(Seq.matchBase(this.seqBuffer.charAt(position), base))
return true;
return false;
}
public static boolean matchBase(char refBase, char base){
char ru = Character.toUpperCase(refBase);
char bu = Character.toUpperCase(base);
boolean returnFlag = false;
if(bu == 'R'){
if(ru == 'A' || ru == 'G')
return true;
}else if(bu == 'Y'){
if(ru == 'C' || ru == 'T')
return true;
}else if(bu == 'S'){
if(ru == 'G' || ru == 'C')
return true;
}else if(bu == 'W'){
if(ru == 'A' || ru == 'T')
return true;
}else if(bu == 'K'){
if(ru == 'G' || ru == 'T')
return true;
}else if(bu == 'M'){
if(ru == 'A' || ru == 'C')
return true;
}else if(bu == 'B'){
if(ru == 'C' || ru == 'G' || ru == 'T')
return true;
}else if(bu == 'D'){
if(ru == 'A' || ru == 'G' || ru == 'T')
return true;
}else if(bu == 'H'){
if(ru == 'A' || ru == 'C' || ru == 'T')
return true;
}else if(bu == 'V'){
if(ru == 'A' || ru == 'C' || ru == 'G')
return true;
}else if(bu == 'N')
return true;
else if(bu == 'A' || bu == 'C' || bu == 'G' || bu == 'T'){
if(ru == bu)
return true;
}
return false;
}
public void printACGT(Gene g){
int[] acgtCounts = this.countACGT(g);
System.out.println(g.getHeader() + "\t" + acgtCounts[0] + "\t" + acgtCounts[1] + "\t" + acgtCounts[2] + "\t" + acgtCounts[3] + "\t" + acgtCounts[4]);
}
public int[] countACGT(Gene g){
int[] acgtCounts = new int[5];
for(int i=g.getStart()-1;i<g.getEnd();i++){
char tmp = seqBuffer.charAt(i);
if(g.fwd()){
if(tmp=='A' || tmp=='a')
acgtCounts[0]++;
else if(tmp == 'C' || tmp == 'c')
acgtCounts[1]++;
else if(tmp == 'G' || tmp == 'g')
acgtCounts[2]++;
else if(tmp == 'T' || tmp == 't')
acgtCounts[3]++;
else
acgtCounts[4]++;
}else{//revcomp
if(tmp=='A' || tmp=='a')
acgtCounts[3]++;
else if(tmp == 'C' || tmp == 'c')
acgtCounts[2]++;
else if(tmp == 'G' || tmp == 'g')
acgtCounts[1]++;
else if(tmp == 'T' || tmp == 't')
acgtCounts[0]++;
else
acgtCounts[4]++;
}
}
//System.out.println(g.getHeader() + "\t" + acgtCounts[0] + "\t" + acgtCounts[1] + "\t" + acgtCounts[2] + "\t" + acgtCounts[3] + "\t" + acgtCounts[4]);
return acgtCounts;
}
public int[] countMotif(String motif, boolean bothStrands){
int[] counts;
if(bothStrands)
counts = new int[2];
else
counts = new int[1];
for(int i=0; i<counts.length;i++){
counts[i] = 0;
}
int i=0;
while(i<this.seqBuffer.length()){
int tmp = this.seqBuffer.indexOf(motif, i);
if(tmp < 0)
i = this.seqBuffer.length();
else{
counts[0]++;
i = tmp + 1;
}
}
if(bothStrands){
StringBuffer rev = revSeqInternal();
i = 0;
while(i<rev.length()){
int tmp = rev.indexOf(motif, i);
if(tmp < 0)
i = this.seqBuffer.length();
else{
counts[1]++;
i = tmp + 1;
}
}
}
return counts;
}
/*
* appendSeq( String ) method appends the sequence
* at the end of the sequence held by Seq obj.
*
*/
public void appendSeq(String tempSeq){
seqBuffer.append(tempSeq);
}
public void insertSeq(int offset, String str){
seqBuffer.insert(offset, str);
}
public void insertSeq(int offset, char c){
seqBuffer.insert(offset, c);
}
public void deleteSeqAt(int offset){
seqBuffer.deleteCharAt(offset);
}
public void replaceSeqAt(int offset, char c){
seqBuffer.replace(offset, offset+1, ""+c);
}
public void replaceSeqAt(int offset, String str){
seqBuffer.replace(offset, offset+1, str);
}
public char seqAt(int index){
return seqBuffer.charAt(index-1);
}
public String getPutationStringForNthBase(int mutTypeIndex, int chosenNthBase, int numLines){
char[] targets = new char[2];
if(mutTypeIndex%2 == 0){ //if an indices is even, it starts from AT, otherwise, it starts from GC
targets[0] = 'A';
targets[1] = 'T';
}else{
targets[0] = 'G';
targets[1] = 'C';
}
int count = 0;
for(int i=0; i<this.seqBuffer.length(); i++){
char curChar = Character.toUpperCase(seqBuffer.charAt(i));
if(curChar == targets[0] || curChar == targets[1]){
//count++;
if(count == chosenNthBase){
return "ecoli " + i + " " + curChar + " " + makePutationArray(curChar, getMutChar(curChar, mutTypeIndex),numLines) + curChar + "\n";
//System.out.println(mutTypeIndex + "\t" + chosenNthBase + "\t" + targets[0]+"/"+targets[1] + "\t" + i);
//return i;
}
count++;
}
}
return null;//return -1;
}
/*
* mutTypeIndex
* 0 , 1 : Transition : AT -> GC, GC -> AT
* 2, 3, 4, 5 : Transversion : AT -> TA, GC -> TA, AT -> CG, GC -> CG
*
*/
public int getIndexForNthBase(int mutTypeIndex, int chosenNthBase, int numLines){
char[] targets = new char[2];
if(mutTypeIndex%2 == 0){ //if an indices is even, it starts from AT, otherwise, it starts from GC
targets[0] = 'A';
targets[1] = 'T';
}else{
targets[0] = 'G';
targets[1] = 'C';
}
int count = 0;
for(int i=0; i<this.seqBuffer.length(); i++){
char curChar = Character.toUpperCase(seqBuffer.charAt(i));
if(curChar == targets[0] || curChar == targets[1]){
//count++;
if(count == chosenNthBase){
System.out.println("ecoli " + i + " " + curChar + " " + makePutationArray(curChar, getMutChar(curChar, mutTypeIndex),numLines) + curChar );
//System.out.println(mutTypeIndex + "\t" + chosenNthBase + "\t" + targets[0]+"/"+targets[1] + "\t" + i);
return i;
}
count++;
}
}
return -1;
}
public int getIndexForNthBase(int mutTypeIndex, int chosenNthBase){
return this.getIndexForNthBase(mutTypeIndex, chosenNthBase, 34);
}
private char getMutChar(char curChar, int mutTypeIndex){
if(mutTypeIndex == 0){
if(curChar == 'A')
return 'G';
else
return 'C';
}else if(mutTypeIndex == 1){
if(curChar == 'G')
return 'A';
else
return 'T';
}else if(mutTypeIndex == 2){
if(curChar == 'A')
return 'T';
else
return 'A';
}else if(mutTypeIndex == 3){
if(curChar == 'G')
return 'T';
else
return 'A';
}else if(mutTypeIndex == 4){
if(curChar == 'A')
return 'C';
else
return 'G';
}else if(mutTypeIndex == 5){
if(curChar == 'G')
return 'C';
else
return 'G';
}else
return 'X';
}
private String makePutationArray(char consChar, char mutChar, int numLines){
StringBuffer bf = new StringBuffer();
int mutLine = (int)(Math.random()*numLines);
for(int i=0; i<numLines;i++){
if(i== mutLine)
bf.append(mutChar + " ");
else
bf.append(consChar + " ");
}
return bf.toString();
}
/*
*
* getSubSeq(int, int) method gets the substring of current seq
* beginIndex is inclusive and end index is exclusive.
*/
public String getSubSeq(int begin, int end){
return seqBuffer.substring(begin-1, end-1);
}
public String getSubSeqWithDelim(int begin, int end, String delim){
String subseq = this.getSubSeq(begin,end);
StringBuffer bf = new StringBuffer();
for(int i=0;i<subseq.length();i++){
bf.append(subseq.charAt(i));
if(i<(subseq.length()-1))
bf.append("\t");
}
return bf.toString();
}
public Seq getSubSeqObj(int begin, int end){
return new Seq(this.seqName, this.getSubSeq(begin, end));
}
public int countGC(){
int count = 0;
for(int i=0; i<this.seqBuffer.length(); i++){
char curChar = seqBuffer.charAt(i);
if(curChar == 'G' || curChar == 'C'
|| curChar == 'g' || curChar == 'c')
count++;
}
return count;
}
/*
* revSeq() method returns the reverse complement sequence of the sequens held by the Seq Obj
*
* @return outBuffer StringBuffer holding the reverse complement seq of the seq held by the Seq Obj
*/
public StringBuffer revSeq(){
StringBuffer tempBuffer = revSeqInternal();
StringBuffer outBuffer = new StringBuffer();
int lowInd = 0;
for(int i=0; i<tempBuffer.length();i++){
if((i % 50) == 49){
outBuffer.append(tempBuffer.substring(lowInd,i+1));
outBuffer.append("\n");
lowInd += 50;
}
}
if( (tempBuffer.length()%50) != 0){
outBuffer.append(tempBuffer.substring(lowInd));
outBuffer.append("\n");
}
return outBuffer;
}
public String getGeneSequenceAndAppendXBases(Gene g, int x){
if(x == 0)
return this.getGeneSequence(g);
int s = g.getStart()-x;
int soff = 0;
int e = g.getEnd()+x;
int eoff = 0;
if(s < 1){
soff = -1*(s) + 1;
s = 1;
}
if(e > this.getSeqLength()){
eoff = e - this.getSeqLength();
e = this.getSeqLength();
}
if(g.getContig().equals(this.seqName)){
String tmp = (soff>0 ? this.getSubSeq(this.getSeqLength()-soff+1,this.getSeqLength()+1) : "")
+ this.getSubSeq(s, e+1)
+ (eoff>0 ? this.getSubSeq(1,1+eoff) : "");
if(g.fwd())
return tmp;
else
return revComp(new StringBuffer(tmp));
}else
return null;
}
public String getGeneSequence(Gene g){
if(g.getContig().equals(this.seqName)){
if(g.fwd())
return this.getSubSeq(g.getStart(), g.getEnd()+1);
else
return revComp(new StringBuffer(this.getSubSeq(g.getStart(), g.getEnd()+1)));
}else
return null;
}
public Seq getGeneSequenceObj(Gene g){
return new Seq(g.getGName(), new StringBuffer(this.getGeneSequence(g)));
}
public static String revComp(StringBuffer sb){
StringBuffer tempBuffer = sb.reverse();
for(int i=0; i<tempBuffer.length();i++){
char curChar = tempBuffer.charAt(i);
tempBuffer.setCharAt(i, rev(curChar));
}
return tempBuffer.toString();
}
private StringBuffer revSeqInternal(){
StringBuffer tempBuffer = seqBuffer.reverse();
for(int i=0; i<tempBuffer.length();i++){
char curChar = tempBuffer.charAt(i);
tempBuffer.setCharAt(i, rev(curChar));
}
return tempBuffer;
}
/*public SynType isSyn(int start, boolean fwd, int position, char mutbase, Codons codons, char consensus){
}*/
public MutType isSyn(int start, boolean fwd, int position, char mutbase, Codons codons){
return this.isSyn(start,fwd,position,mutbase,codons,Character.toUpperCase(seqBuffer.charAt(position-1)));
}
/*
* Changed to carry mutType information
*
*/
public MutType isSyn(int start, boolean fwd, int position, char mutbase, Codons codons, char consensus){
try{
//System.out.print(mutbase + " ");
mutbase = Character.toUpperCase(mutbase);
//if(mutbase == consensus)
// return 1;
//else{
int codonPos = this.getStartOfGene(start,fwd,position);
StringBuffer triple = new StringBuffer();
StringBuffer refTriple = new StringBuffer();
if(fwd){
if(codonPos == 0){
refTriple.append(consensus);
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position)));
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position+1)));
triple.append(mutbase);
triple.append(Character.toUpperCase(seqBuffer.charAt(position)));
triple.append(Character.toUpperCase(seqBuffer.charAt(position+1)));
}else if(codonPos == 1){
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position-2)));
refTriple.append(consensus);
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position)));
triple.append(Character.toUpperCase(seqBuffer.charAt(position-2)));
triple.append(mutbase);
triple.append(Character.toUpperCase(seqBuffer.charAt(position)));
}else if(codonPos == 2){
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position-3)));
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position-2)));
refTriple.append(consensus);
triple.append(Character.toUpperCase(seqBuffer.charAt(position-3)));
triple.append(Character.toUpperCase(seqBuffer.charAt(position-2)));
triple.append(mutbase);
}
}else{//reverse
if(codonPos == 0){
refTriple.append(rev(consensus));
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-2))));
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-3))));
triple.append(rev(mutbase));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-2))));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-3))));
}else if(codonPos == 1){
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position))));
refTriple.append(rev(consensus));
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-2))));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position))));
triple.append(rev(mutbase));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-2))));
}else if(codonPos == 2){
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position+1))));
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position))));
refTriple.append(rev(consensus));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position+1))));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position))));
triple.append(rev(mutbase));
}
}
return codons.isSyn(refTriple.toString(), triple.toString());
//}
}catch(Exception e){
System.err.println("|" + start + "\t" + fwd + "\t" + position + "\t" + mutbase +"\t" +codons);//t start, boolean fwd, int position, char mutbase, Codons codons);
//System.err.println(ct.toStringBuffer().toString());
e.printStackTrace();
System.exit(1);
}
return null;
}
/*
* start: in case of (-) stranded gene, start here is the actual start --> so 2nd column of fraggenescan output
* in other words, start is always the 5' end of the gene(start position of the gene)
*
* RETURN 1 if syn, 0 if non-synonymous, -1 if codon contains bases other than a t g c
*/
/*public int isSyn(int start, boolean fwd, int position, char mutbase, Codons codons, char consensus){
try{
//System.out.print(mutbase + " ");
mutbase = Character.toUpperCase(mutbase);
if(mutbase == consensus)
return 1;
else{
int codonPos = this.getStartOfGene(start,fwd,position);
StringBuffer triple = new StringBuffer();
StringBuffer refTriple = new StringBuffer();
if(fwd){
if(codonPos == 0){
refTriple.append(consensus);
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position)));
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position+1)));
triple.append(mutbase);
triple.append(Character.toUpperCase(seqBuffer.charAt(position)));
triple.append(Character.toUpperCase(seqBuffer.charAt(position+1)));
}else if(codonPos == 1){
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position-2)));
refTriple.append(consensus);
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position)));
triple.append(Character.toUpperCase(seqBuffer.charAt(position-2)));
triple.append(mutbase);
triple.append(Character.toUpperCase(seqBuffer.charAt(position)));
}else if(codonPos == 2){
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position-3)));
refTriple.append(Character.toUpperCase(seqBuffer.charAt(position-2)));
refTriple.append(consensus);
triple.append(Character.toUpperCase(seqBuffer.charAt(position-3)));
triple.append(Character.toUpperCase(seqBuffer.charAt(position-2)));
triple.append(mutbase);
}
}else{//reverse
if(codonPos == 0){
refTriple.append(rev(consensus));
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-2))));
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-3))));
triple.append(rev(mutbase));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-2))));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-3))));
}else if(codonPos == 1){
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position))));
refTriple.append(rev(consensus));
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-2))));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position))));
triple.append(rev(mutbase));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position-2))));
}else if(codonPos == 2){
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position+1))));
refTriple.append(rev(Character.toUpperCase(seqBuffer.charAt(position))));
refTriple.append(rev(consensus));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position+1))));
triple.append(rev(Character.toUpperCase(seqBuffer.charAt(position))));
triple.append(rev(mutbase));
}
}
return codons.isSyn(refTriple.toString(), triple.toString());
}
}catch(Exception e){
System.err.println("|" + start + "\t" + fwd + "\t" + position + "\t" + mutbase +"\t" +codons);//t start, boolean fwd, int position, char mutbase, Codons codons);
//System.err.println(ct.toStringBuffer().toString());
e.printStackTrace();
System.exit(1);
}
return 0;
}
*/
private static char rev(char curChar){
if(curChar == 'T')
return 'A';
else if(curChar == 't')
return 'a';
else if(curChar == 'A')
return 'T';
else if(curChar == 'a')
return 't';
else if(curChar == 'G')
return 'C';
else if(curChar == 'g')
return 'c';
else if(curChar == 'C')
return 'G';
else if(curChar == 'c')
return 'g';
else
return curChar;
}
private String getCodon(boolean fwd, int codonPos, int position, char mutbase){
if(codonPos == 0)
;
return new String();
}
/*
*
* @param
* start --> start position of target gene
* fwd --> true if fwd, rev otherwise
* position --> position of polymorphism
*
* @returns
* integer value of codon position for the given polymorphic pos.
* 0 --> 1st pos of triplet
* 1 --> 2nd pos of triplet
* 2 --> 3rd pos of triplet