diff --git a/ddtrace/debugging/_probe/registry.py b/ddtrace/debugging/_probe/registry.py
index 32e8f443ea6..ec8e1239804 100644
--- a/ddtrace/debugging/_probe/registry.py
+++ b/ddtrace/debugging/_probe/registry.py
@@ -150,10 +150,10 @@ def log_probes_status(self) -> None:
                 self._log_probe_status_unlocked(entry)
 
     def _remove_pending(self, probe: Probe) -> None:
-        location = _get_probe_location(probe)
-
-        # Pending probes must have valid location information
-        assert location is not None, probe  # nosec
+        if (location := _get_probe_location(probe)) is None:
+            # If the probe has no location information, then it cannot be
+            # pending.
+            return
 
         pending_probes = self._pending[location]
         try:
diff --git a/releasenotes/notes/fix-unresolved-pending-probe-removal-80fbf85a9c068122.yaml b/releasenotes/notes/fix-unresolved-pending-probe-removal-80fbf85a9c068122.yaml
new file mode 100644
index 00000000000..64356aed8d9
--- /dev/null
+++ b/releasenotes/notes/fix-unresolved-pending-probe-removal-80fbf85a9c068122.yaml
@@ -0,0 +1,5 @@
+---
+fixes:
+  - |
+    dynamic instrumentation: prevent an exception when trying to remove a probe
+    that did not resolve to a valid source code location.
diff --git a/tests/debugging/probe/test_registry.py b/tests/debugging/probe/test_registry.py
index 49819f315ed..fd9aff4d024 100644
--- a/tests/debugging/probe/test_registry.py
+++ b/tests/debugging/probe/test_registry.py
@@ -56,6 +56,9 @@ def test_registry_location_error():
     # Check that the probe is not pending
     assert not registry.get_pending(__file__)
 
+    # Check that unregistering the probe does not cause an exception
+    registry.unregister(probe)
+
     # Check that we emitted the correct diagnostic error message
     for e in status_logger.queue:
         del e["timestamp"]