Skip to content

hisat2默认流程下处理的bam文件不能用gtz压缩 #21

@boiscat

Description

@boiscat

ref文件:genome.fa (hg19)
下载地址:https://hgdownload.soe.ucsc.edu/goldenPath/hg19/bigZips/chromFa.tar.gz

hisat2运行的ref: hg19 genome_tran
下载地址:https://ccb.jhu.edu/software/hisat2/manual.shtml

已经通过默认的hisat2流程生成bam文件(没有用hisat2-gtz),gtz打包时报错如下:


prepare compression... 100%, cost 23s (18|5)
Enabling high-rate compression mode with /home/.config/gtz/genome.fa-D2A70550489DE356A2CD6BFC40711204.bam.rbin2( hardware speedup )
[                                                  ] 0%

RNAME "1" was not found in reference file: /home/Erythropoiesis_APA/HSC_APA/hsc_apa1/04-salmon/00-file/genome.fa
error: the reference file was detected to not match this BAM file!

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type
    No fields configured for issues without a type.

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions