From fa5255f43a3eeed2fad753e0492b16e3ef754fbb Mon Sep 17 00:00:00 2001 From: rclarke0 Date: Tue, 7 Jan 2025 09:42:14 -0500 Subject: [PATCH 1/2] remove liquid waste emptying, change version no., change default # of runs --- .../6_Omega_HDQ_DNA_Cells-Flex_96_channel.py | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py b/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py index 894f80dcdea..92bfb1e32dc 100644 --- a/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py +++ b/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py @@ -33,7 +33,7 @@ def add_parameters(parameters: ParameterContext) -> None: parameters.add_int( variable_name="number_of_runs", display_name="Number of Runs", - default=2, + default=4, minimum=1, maximum=4, ) @@ -53,7 +53,7 @@ def run(protocol: ProtocolContext) -> None: bind_vol = 300.0 sample_vol = 180.0 elution_vol = 100.0 - helpers.comment_protocol_version(protocol, "01") + helpers.comment_protocol_version(protocol, "02") # Same for all HDQ Extractions deepwell_type = "nest_96_wellplate_2ml_deep" if not dry_run: @@ -417,7 +417,6 @@ def clean() -> None: elutionplate, wash2_reservoir, wash1_reservoir, - liquid_waste, ] helpers.clean_up_plates(pip, plates_to_clean, liquid_waste["A1"], 1000) From 7afb347bbdc14ba80867fc359fa0798c5bad288d Mon Sep 17 00:00:00 2001 From: rclarke0 Date: Fri, 10 Jan 2025 14:29:43 -0500 Subject: [PATCH 2/2] change reservoir to 290 ml --- .../active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py b/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py index 92bfb1e32dc..a3fd6251a43 100644 --- a/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py +++ b/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py @@ -87,7 +87,7 @@ def run(protocol: ProtocolContext) -> None: magblock: MagneticBlockContext = protocol.load_module( "magneticBlockV1", "C1" ) # type: ignore[assignment] - liquid_waste = protocol.load_labware("nest_1_reservoir_195ml", "B3", "Liquid Waste") + liquid_waste = protocol.load_labware("nest_1_reservoir_290ml", "B3", "Liquid Waste") waste = liquid_waste.wells()[0].top() lysis_reservoir = protocol.load_labware(deepwell_type, "D2", "Lysis reservoir")