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@@ -33,6 +33,22 @@ check the [S3 entry](https://bbglab.github.io/bbgwiki/Cluster_basics/s3/#termina
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## Metrics
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These are a set of metrics that help us understand two key aspects: whether our duplex libraries have properly worked and importantly to estimate how much sequencing output should be requested to avoid undersequencing but most importantly oversequencing.
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*When should I run metrics*
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Everytime you do a new deepUMIcaller run and before running deepCSA.
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*Why?*
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1. To validate you have included all GBs of data available for that library (all lanes and reseqs)
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2. To check whether you library has been sequenced to optimal or additional reseq needs to be requested
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3. To continue the effort of compiling these metrics to keep improving our understanding of the duplex protocol
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*How*
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You can find the instructions on how to run them and additional documentation on metrics in our internal duplex documentation.
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## deepCSA
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We use the code available in [deepCSA](https://github.com/bbglab/deepCSA.git), we generally use the dev branch
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