diff --git a/src/Servers/IIS/IIS/test/IIS.Tests/TlsHandshakeFeatureTests.cs b/src/Servers/IIS/IIS/test/IIS.Tests/TlsHandshakeFeatureTests.cs
index 7be0fbe6b98d..958c3b24a4ec 100644
--- a/src/Servers/IIS/IIS/test/IIS.Tests/TlsHandshakeFeatureTests.cs
+++ b/src/Servers/IIS/IIS/test/IIS.Tests/TlsHandshakeFeatureTests.cs
@@ -13,7 +13,6 @@ namespace Microsoft.AspNetCore.Server.IIS.FunctionalTests;
 public class TlsHandshakeFeatureTests : StrictTestServerTests
 {
     [ConditionalFact]
-    [QuarantinedTest("https://github.com/dotnet/aspnetcore/issues/49306")]
     public async Task SetsTlsHandshakeFeatureForHttps()
     {
         ITlsHandshakeFeature tlsHandshakeFeature = null;