diff --git a/eng/pipelines/extra-platforms/runtime-extra-platforms-ioslike.yml b/eng/pipelines/extra-platforms/runtime-extra-platforms-ioslike.yml
index 9a769124a05dcf..c123ea162e4d08 100644
--- a/eng/pipelines/extra-platforms/runtime-extra-platforms-ioslike.yml
+++ b/eng/pipelines/extra-platforms/runtime-extra-platforms-ioslike.yml
@@ -39,9 +39,9 @@ jobs:
       isExtraPlatforms: ${{ parameters.isExtraPlatformsBuild }}
       # Don't trim tests on rolling builds
       ${{ if eq(variables['isRollingBuild'], true) }}:
-        buildArgs: -s mono+libs+libs.tests -c $(_BuildConfig) /p:ArchiveTests=true /p:DevTeamProvisioning=- /p:RunAOTCompilation=true $(_runSmokeTestsOnlyArg) /p:BuildTestsOnHelix=true /p:EnableAdditionalTimezoneChecks=true /p:UsePortableRuntimePack=true /p:BuildDarwinFrameworks=true /p:IsManualOrRollingBuild=true /p:EnableAggressiveTrimming=false
+        buildArgs: -s mono+libs+libs.tests -c $(_BuildConfig) /p:ArchiveTests=true /p:DevTeamProvisioning=- /p:RunAOTCompilation=true $(_runSmokeTestsOnlyArg) /p:BuildTestsOnHelix=true /p:EnableAdditionalTimezoneChecks=true /p:UsePortableRuntimePack=false /p:BuildDarwinFrameworks=true /p:IsManualOrRollingBuild=true /p:EnableAggressiveTrimming=false
       ${{ else }}:
-        buildArgs: -s mono+libs+libs.tests -c $(_BuildConfig) /p:ArchiveTests=true /p:DevTeamProvisioning=- /p:RunAOTCompilation=true $(_runSmokeTestsOnlyArg) /p:BuildTestsOnHelix=true /p:EnableAdditionalTimezoneChecks=true /p:UsePortableRuntimePack=true /p:BuildDarwinFrameworks=true /p:IsManualOrRollingBuild=true /p:EnableAggressiveTrimming=true
+        buildArgs: -s mono+libs+libs.tests -c $(_BuildConfig) /p:ArchiveTests=true /p:DevTeamProvisioning=- /p:RunAOTCompilation=true $(_runSmokeTestsOnlyArg) /p:BuildTestsOnHelix=true /p:EnableAdditionalTimezoneChecks=true /p:UsePortableRuntimePack=false /p:BuildDarwinFrameworks=true /p:IsManualOrRollingBuild=true /p:EnableAggressiveTrimming=true
       timeoutInMinutes: 480
       # extra steps, run tests
       postBuildSteps:
diff --git a/eng/pipelines/runtime.yml b/eng/pipelines/runtime.yml
index 49f615c82c5d23..a7b03da8ddd183 100644
--- a/eng/pipelines/runtime.yml
+++ b/eng/pipelines/runtime.yml
@@ -973,7 +973,7 @@ extends:
           jobParameters:
             testGroup: innerloop
             nameSuffix: AllSubsets_Mono
-            buildArgs: -s mono+libs+libs.tests+host+packs -c $(_BuildConfig) /p:ArchiveTests=true /p:DevTeamProvisioning=- /p:RunAOTCompilation=true /p:RunSmokeTestsOnly=true /p:BuildTestsOnHelix=true /p:EnableAdditionalTimezoneChecks=true /p:UsePortableRuntimePack=true /p:BuildDarwinFrameworks=true /p:EnableAggressiveTrimming=true
+            buildArgs: -s mono+libs+libs.tests+host+packs -c $(_BuildConfig) /p:ArchiveTests=true /p:DevTeamProvisioning=- /p:RunAOTCompilation=true /p:RunSmokeTestsOnly=true /p:BuildTestsOnHelix=true /p:EnableAdditionalTimezoneChecks=true /p:UsePortableRuntimePack=false /p:BuildDarwinFrameworks=true /p:EnableAggressiveTrimming=true
             timeoutInMinutes: 480
             condition: >-
               or(
diff --git a/eng/testing/tests.ioslike.targets b/eng/testing/tests.ioslike.targets
index a59cce6c49aeea..e2e36415a31834 100644
--- a/eng/testing/tests.ioslike.targets
+++ b/eng/testing/tests.ioslike.targets
@@ -16,7 +16,7 @@
     <!-- running aot-helix tests locally, so we can test with the same project file as CI -->
     <_AOTBuildCommand Condition="'$(ContinuousIntegrationBuild)' != 'true'">$(_AOTBuildCommand) /p:RuntimeSrcDir=$(RepoRoot) /p:RuntimeConfig=$(Configuration)</_AOTBuildCommand>
     <!-- The command below sets default properties for runtime and library tests -->
-    <_AOTBuildCommand>$(_AOTBuildCommand) /p:XHARNESS_EXECUTION_DIR=&quot;$XHARNESS_EXECUTION_DIR&quot; /p:RunAOTCompilation=$(RunAOTCompilation) /p:UseNativeAOTRuntime=$(UseNativeAOTRuntime) /p:TargetOS=$(TargetOS) /p:TargetArchitecture=$(TargetArchitecture) /p:MonoForceInterpreter=$(MonoForceInterpreter) /p:MonoEnableLLVM=true /p:DevTeamProvisioning=$(DevTeamProvisioning) /p:UsePortableRuntimePack=true /p:Configuration=$(Configuration) /p:EnableAggressiveTrimming=$(EnableAggressiveTrimming)</_AOTBuildCommand>
+    <_AOTBuildCommand>$(_AOTBuildCommand) /p:XHARNESS_EXECUTION_DIR=&quot;$XHARNESS_EXECUTION_DIR&quot; /p:RunAOTCompilation=$(RunAOTCompilation) /p:UseNativeAOTRuntime=$(UseNativeAOTRuntime) /p:TargetOS=$(TargetOS) /p:TargetArchitecture=$(TargetArchitecture) /p:MonoForceInterpreter=$(MonoForceInterpreter) /p:MonoEnableLLVM=true /p:DevTeamProvisioning=$(DevTeamProvisioning) /p:UsePortableRuntimePack=$(UsePortableRuntimePack) /p:Configuration=$(Configuration)</_AOTBuildCommand>
     <_AOTBuildCommand Condition="'$(NativeLib)' != ''">$(_AOTBuildCommand) /p:NativeLib=$(NativeLib) /p:BundlesResources=$(BundlesResources) /p:ForceLibraryModeGenerateAppBundle=$(ForceLibraryModeGenerateAppBundle)</_AOTBuildCommand>
     <_AOTBuildCommand>$(_AOTBuildCommand) </_AOTBuildCommand>
 
@@ -77,8 +77,6 @@
       <BundleFiles Include="$(MonoProjectRoot)\msbuild\apple\data\*" TargetDir="publish" />
       <ExtraFiles Condition="'%(AppleAssembliesToBundle._IsNative)' == 'true'"
                    Include="@(AppleAssembliesToBundle)"          TargetDir="extraFiles\%(AppleAssembliesToBundle.RecursiveDir)" />
-      <ExtraFiles Condition="Exists(%(TrimmerRootDescriptor.Identity))"
-                   Include="@(TrimmerRootDescriptor)"            TargetDir="extraFiles" />
     </ItemGroup>
 
     <ItemGroup Condition="'$(UseNativeAOTRuntime)' == 'true' and '$(HybridGlobalization)' == 'false'" >
@@ -156,12 +154,6 @@
       <_AppleItemsToPass Include="@(ReferenceExtraPathFiles->'%(FileName)%(Extension)')"
                          OriginalItemName__="AppleReferenceExtraPathFiles" />
 
-      <_AppleItemsToPass Include="@(RuntimeHostConfigurationOption)"
-                         OriginalItemName__="_AppleUsedRuntimeHostConfigurationOption" />
-
-      <_AppleItemsToPass Include="@(TrimmerRootAssembly)"
-                         OriginalItemName__="TrimmerRootAssembly" />
-
       <!-- Example of passing items to the project
 
           <_AppleItemsToPass Include="@(BundleFiles)" OriginalItemName__="BundleFiles" ConditionToUse__="'$(Foo)' != 'true'" />
diff --git a/eng/testing/tests.mobile.targets b/eng/testing/tests.mobile.targets
index 2cc3731b068967..685c3b98aff181 100644
--- a/eng/testing/tests.mobile.targets
+++ b/eng/testing/tests.mobile.targets
@@ -24,24 +24,20 @@
     <DotnetPgoToolPath>$([MSBuild]::NormalizePath('$(DotnetPgoToolDir)', 'dotnet-pgo'))</DotnetPgoToolPath>
   </PropertyGroup>
 
-  <!-- When tests are built on Helix, we don't want to invoke ILLink on build machines -->
-  <PropertyGroup Condition="'$(EnableAggressiveTrimming)' == 'true' and '$(BuildTestsOnHelix)' != 'true'">
+  <!-- Invoke ILLink on build machines to match ILLink features -->
+  <PropertyGroup Condition="'$(EnableAggressiveTrimming)' == 'true'">
     <PublishTrimmed>true</PublishTrimmed>
     <!-- Suppress trimming warnings as these are tests -->
     <SuppressTrimAnalysisWarnings>true</SuppressTrimAnalysisWarnings>
     <NoWarn>$(NoWarn);IL2103;IL2025;IL2111;IL2122</NoWarn>
 
     <!-- Reduce library test app size by trimming framework library features  -->
-    <DebuggerSupport Condition="'$(DebuggerSupport)' == '' and '$(Configuration)' != 'Debug'">false</DebuggerSupport>
     <EnableUnsafeUTF7Encoding Condition="'$(EnableUnsafeUTF7Encoding)' == ''">false</EnableUnsafeUTF7Encoding>
-    <EventSourceSupport Condition="'$(EventSourceSupport)' == ''">false</EventSourceSupport>
-    <HttpActivityPropagationSupport Condition="'$(HttpActivityPropagationSupport)' == ''">false</HttpActivityPropagationSupport>
     <UseSystemResourceKeys Condition="'$(UseSystemResourceKeys)' == ''">false</UseSystemResourceKeys>
-    <UseNativeHttpHandler Condition="'$(UseNativeHttpHandler)' == ''">false</UseNativeHttpHandler>
   </PropertyGroup>
 
-  <!-- When tests are built on Helix, override default trimming switches  -->
-  <PropertyGroup Condition="'$(EnableAggressiveTrimming)' == 'true' and '$(BuildTestsOnHelix)' == 'true'">
+  <!-- Override default trimming switches for Apple mobile -->
+  <PropertyGroup Condition="'$(EnableAggressiveTrimming)' == 'true' and '$(TargetsAppleMobile)' == 'true'">
     <DynamicCodeSupport Condition="'$(DynamicCodeSupport)' == '' and '$(MonoForceInterpreter)' != 'true'">false</DynamicCodeSupport>
     <_DefaultValueAttributeSupport Condition="'$(OverrideDefaultValueAndDesignerHostSupport)' == 'true'">true</_DefaultValueAttributeSupport>
     <_DesignerHostSupport Condition="'$(OverrideDefaultValueAndDesignerHostSupport)' == 'true'">true</_DesignerHostSupport>
diff --git a/src/libraries/Microsoft.Extensions.DependencyModel/tests/Microsoft.Extensions.DependencyModel.Tests.csproj b/src/libraries/Microsoft.Extensions.DependencyModel/tests/Microsoft.Extensions.DependencyModel.Tests.csproj
index 7717367bbbbe14..70fd9e556fb285 100644
--- a/src/libraries/Microsoft.Extensions.DependencyModel/tests/Microsoft.Extensions.DependencyModel.Tests.csproj
+++ b/src/libraries/Microsoft.Extensions.DependencyModel/tests/Microsoft.Extensions.DependencyModel.Tests.csproj
@@ -18,4 +18,7 @@
     <ProjectReference Include="nonentrypointassembly\nonentrypointassembly.csproj" />
   </ItemGroup>
 
+  <ItemGroup Condition="'$(TargetsAppleMobile)' == 'true' and '$(EnableAggressiveTrimming)' == 'true' and '$(UseNativeAotRuntime)' != 'true'" >
+    <TrimmerRootAssembly Include="nonentrypointassembly" />
+  </ItemGroup>
 </Project>
diff --git a/src/libraries/System.Reflection.TypeExtensions/tests/ILLink.Descriptors.xml b/src/libraries/System.Reflection.TypeExtensions/tests/ILLink.Descriptors.xml
deleted file mode 100644
index 573b67e7172d49..00000000000000
--- a/src/libraries/System.Reflection.TypeExtensions/tests/ILLink.Descriptors.xml
+++ /dev/null
@@ -1,3 +0,0 @@
-<linker>
-  <assembly fullname="TinyAssembly" />
-</linker>
diff --git a/src/libraries/System.Reflection.TypeExtensions/tests/System.Reflection.TypeExtensions.Tests.csproj b/src/libraries/System.Reflection.TypeExtensions/tests/System.Reflection.TypeExtensions.Tests.csproj
index 4cec27f2eab318..eec6c3be0d0e89 100644
--- a/src/libraries/System.Reflection.TypeExtensions/tests/System.Reflection.TypeExtensions.Tests.csproj
+++ b/src/libraries/System.Reflection.TypeExtensions/tests/System.Reflection.TypeExtensions.Tests.csproj
@@ -22,6 +22,5 @@
       <Link>TinyAssembly.dll</Link>
       <CopyToOutputDirectory>PreserveNewest</CopyToOutputDirectory>
     </Content>
-    <TrimmerRootDescriptor Condition="'$(TargetsAppleMobile)' == 'true' and '$(EnableAggressiveTrimming)' == 'true' and '$(UseNativeAotRuntime)' != 'true'" Include="$(MSBuildThisFileDirectory)ILLink.Descriptors.xml" />
   </ItemGroup>
 </Project>
diff --git a/src/libraries/System.Runtime/tests/System.Runtime.Tests/ILLink.Descriptors.iOS.xml b/src/libraries/System.Runtime/tests/System.Runtime.Tests/ILLink.Descriptors.iOS.xml
index b327a37c7a60be..9f5adb4befda83 100644
--- a/src/libraries/System.Runtime/tests/System.Runtime.Tests/ILLink.Descriptors.iOS.xml
+++ b/src/libraries/System.Runtime/tests/System.Runtime.Tests/ILLink.Descriptors.iOS.xml
@@ -9,11 +9,8 @@
     <type fullname="System.Tests.EnumTests" />
     <type fullname="System.Tests.ArrayTests" />
     <type fullname="System.Reflection.Tests.InvokeRefReturnNetcoreTests" />
-    <type fullname="System.Reflection.Tests.InvokeRefReturnNetcoreTests+TestClass`1" />
     <type fullname="System.Reflection.Tests.MethodBaseTests" />
-    <type fullname="System.Tests.Types.ModifiedTypeTests+ModifiedTypeHolder" />
     <type fullname="System.Tests.ArrayTests" />
-    <type fullname="System.Tests.ArrayTests+GenericStruct`1" />
   </assembly>
   <assembly fullname="System.Private.CoreLib">
     <type fullname="System.Array" />
diff --git a/src/libraries/tests.proj b/src/libraries/tests.proj
index fae36df0a3f5b1..76f22763eac181 100644
--- a/src/libraries/tests.proj
+++ b/src/libraries/tests.proj
@@ -339,6 +339,10 @@
     <ProjectExclusions Include="$(MSBuildThisFileDirectory)System.Net.Security/tests/FunctionalTests/System.Net.Security.Tests.csproj" />
     <!-- Reference deleted by _CopyTestArchive https://github.com/dotnet/runtime/issues/80976 -->
     <ProjectExclusions Include="$(RepoRoot)/src/tests/FunctionalTests/iOS/Simulator/StartupHook/iOS.Simulator.StartupHook.Test.csproj" />
+    <!-- Tracking issue: https://github.com/dotnet/runtime/issues/101692 -->
+    <ProjectExclusions Include="$(MSBuildThisFileDirectory)System.Formats.Tar/tests/System.Formats.Tar.Tests.csproj" />
+    <!-- Tracking issue: https://github.com/dotnet/runtime/issues/109282 -->
+    <ProjectExclusions Include="$(MSBuildThisFileDirectory)System.Runtime/tests/System.IO.UnmanagedMemoryStream.Tests/System.IO.UnmanagedMemoryStream.Tests.csproj" />
 
     <!-- Source generators tests. Not testing customer scenarios. Low priority -->
     <ProjectExclusions Include="$(MSBuildThisFileDirectory)Microsoft.Extensions.Logging.Abstractions\tests\Microsoft.Extensions.Logging.Generators.Tests\Microsoft.Extensions.Logging.Generators.Roslyn3.11.Tests.csproj" />
diff --git a/src/mono/msbuild/apple/data/ProxyProjectForAOTOnHelix.proj b/src/mono/msbuild/apple/data/ProxyProjectForAOTOnHelix.proj
index 2847f567f469a1..2035333122a2f0 100644
--- a/src/mono/msbuild/apple/data/ProxyProjectForAOTOnHelix.proj
+++ b/src/mono/msbuild/apple/data/ProxyProjectForAOTOnHelix.proj
@@ -7,7 +7,6 @@
     <ExtraFilesPath>$([MSBuild]::NormalizeDirectory($(TestRootDir), '..', 'extraFiles'))</ExtraFilesPath>
     <BaseIntermediateOutputPath>$([MSBuild]::NormalizeDirectory($(TestRootDir), '..', 'obj'))</BaseIntermediateOutputPath>
 
-    <AppleBuildDependsOn Condition="'$(EnableAggressiveTrimming)' == 'true' and '$(UseNativeAOTRuntime)' != 'true'">ConfigureTrimming;_AdjustTrimmedAssembliesToBundle;$(AppleBuildDependsOn)</AppleBuildDependsOn>
     <AppleBuildDependsOn>_PublishRuntimePack;_PrepareForAppleBuildAppOnHelix;$(AppleBuildDependsOn);_AfterAppleBuildOnHelix</AppleBuildDependsOn>
     <!-- Forced by ILLink targets -->
     <SelfContained>true</SelfContained>
@@ -71,7 +70,7 @@
 
     <ItemGroup>
       <!-- Figure out if we can support JustInterp mode -->
-      <AppleAssembliesToBundle Include="$(OriginalPublishDir)**\*.dll" Exclude="$(OriginalPublishDir)\**\*.resources.dll" />
+    <AppleAssembliesToBundle Include="$(OriginalPublishDir)\*.dll" Exclude="$(OriginalPublishDir)\*.resources.dll" />
 
       <!-- Extra files are NativeLibraries and should be excluded from AppleAssembliesToBundle -->
       <_ExtraFiles Include="$(ExtraFilesPath)**\*" />
@@ -95,14 +94,6 @@
       <RuntimeComponents Include="diagnostics_tracing" />
       <RuntimeComponents Include="marshal-ilgen" />
     </ItemGroup>
-
-    <ItemGroup Condition="'$(EnableAggressiveTrimming)' == 'true' and '$(UseNativeAOTRuntime)' != 'true'">
-       <TrimmerRootDescriptor Include="$(ExtraFilesPath)**\*.xml" />
-
-       <!-- To recreate the state of feature flags (RuntimeHostConfigurationOption) during app build-time we need to discard the default setting, coming from SDK, and used the values passed in ProxyProjectForAOTOnHelix.props -->
-       <RuntimeHostConfigurationOption Remove="@(_AppleUsedRuntimeHostConfigurationOption)" />
-       <RuntimeHostConfigurationOption Include="@(_AppleUsedRuntimeHostConfigurationOption)" />
-    </ItemGroup>
   </Target>
 
   <Target Name="_PublishRuntimePack"
@@ -117,14 +108,6 @@
           DestinationFolder="$(OriginalPublishDir)" />
   </Target>
 
-  <!-- Since we are reusing the 'publish' folder for different purposes (e.g., using it as a source and destination directory for trimming) we need to adjust the list of assemblies to bundle once trimming is completed. -->
-  <Target Name="_AdjustTrimmedAssembliesToBundle" DependsOnTargets="ILLink;CopyFilesToPublishDirectory">
-    <ItemGroup>
-      <AppleAssembliesToBundle Remove="@(AppleAssembliesToBundle)" />
-      <AppleAssembliesToBundle Include="$(OriginalPublishDir)**\*.dll" Exclude="$(OriginalPublishDir)\**\*.resources.dll;@(_RemovedManagedAssembly)" />
-    </ItemGroup>
-  </Target>
-
   <!-- Move the app to where the xharness runner expects it. -->
   <Target Name="_AfterAppleBuildOnHelix">
     <PropertyGroup>
diff --git a/src/tests/FunctionalTests/tvOS/Device/AOT/tvOS.Device.Aot.Test.csproj b/src/tests/FunctionalTests/tvOS/Device/AOT/tvOS.Device.Aot.Test.csproj
index 0e2f71fe06b3d0..92271eb0c889ce 100644
--- a/src/tests/FunctionalTests/tvOS/Device/AOT/tvOS.Device.Aot.Test.csproj
+++ b/src/tests/FunctionalTests/tvOS/Device/AOT/tvOS.Device.Aot.Test.csproj
@@ -19,11 +19,6 @@
     <MonoForceInterpreter>false</MonoForceInterpreter>
   </PropertyGroup>
 
-  <ItemGroup>
-    <!-- Preserves the UnmanagedCallersOnly method -->
-    <TrimmerRootDescriptor Include="$(MSBuildThisFileDirectory)ILLink.Descriptors.xml" />
-  </ItemGroup>
-
   <ItemGroup>
     <Compile Include="Program.cs" />
   </ItemGroup>