seqan::FileOpen Error' Could not open file : iostream error

[mrb20045]

Hi
When I ran the below code, I get error and dont know how can I fix it. Could you please check it ?

popins2 place-splitalign -c All_Assemblies.fa -r nut_2.0.fa sample1

[popins2 2023-06-02 12:05:49] Reading locations from ./sample1/locations_unplaced.txt
[popins2 2023-06-02 12:05:50] Loaded 123184 locations that pass filters.
[popins2 2023-06-02 12:05:50] Reading contig sequences from All_Assemblies.fa
[popins2 2023-06-02 12:05:58] Loaded 78356 contig sequences.
terminate called after throwing an instance of 'seqan::FileOpenError'
what(): Could not open file : iostream error
Aborted

Thanks

[Krannich479]

hi @mrb20045 ,
given your output, the error message and the message that should follow in case of a successful run of popins2 place-splitalign, let's investigate the following things, in that order:

1. Did a BAM+BAI file exist within the sample1 folder at the time you ran the command?
2. Do you have write permissions on the sample1 folder?

One more general question: is this a re-run or first time execution of popins on that sample?