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I have an impression that sequences of the Illumina TruSeq adaptors starting from Index 13 are wrong. I checked in couple sources, including Illumina website, and starting from Index 13 sequences are different, for example
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAC TCTCGTATGCCGTCTTCTGCTTG # Index 13, current contaminant_list.txt
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTG # Index 13, Illumina file
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCG TCTCGTATGCCGTCTTCTGCTTG # Index 14, current contaminant_list.txt
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTG # Index 14, Illumina file
(I have added spaces to align easier) You can see that after the 6-letter barcode, there is a 2-letter difference.
Additionally, for Index 23 (
I have an impression that sequences of the Illumina TruSeq adaptors starting from Index 13 are wrong. I checked in couple sources, including Illumina website, and starting from Index 13 sequences are different, for example
(I have added spaces to align easier) You can see that after the 6-letter barcode, there is a 2-letter difference.
Additionally, for Index 23 (
FastQC/Configuration/contaminant_list.txt
Line 114 in 45c9977
Overall, maybe it would be helpful to indicate source of information as a comment line before each block of sequences?
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