diff --git a/requirements/REQUIREMENTS-CI.txt b/requirements/REQUIREMENTS-CI.txt
index 79ee88ef..59033586 100644
--- a/requirements/REQUIREMENTS-CI.txt
+++ b/requirements/REQUIREMENTS-CI.txt
@@ -142,7 +142,7 @@ stack-data==0.6.3
 sympy==1.14.0
 tabulate==0.9.0
 threadpoolctl==3.6.0
-tifffile==2024.8.30
+tifffile==2025.10.16
 tinycss2==1.4.0
 tomli==2.3.0
 tornado==6.5.2
diff --git a/requirements/REQUIREMENTS-JUPYTER.txt b/requirements/REQUIREMENTS-JUPYTER.txt
index 6e1820cc..ad9f4832 100644
--- a/requirements/REQUIREMENTS-JUPYTER.txt
+++ b/requirements/REQUIREMENTS-JUPYTER.txt
@@ -125,7 +125,7 @@ stack-data==0.6.3
 sympy==1.14.0
 terminado==0.18.1
 threadpoolctl==3.6.0
-tifffile==2024.8.30
+tifffile==2025.10.16
 tinycss2==1.4.0
 tomli==2.3.0
 tornado==6.5.2
diff --git a/requirements/REQUIREMENTS-NAPARI-CI.txt b/requirements/REQUIREMENTS-NAPARI-CI.txt
index 2539d272..5c290456 100644
--- a/requirements/REQUIREMENTS-NAPARI-CI.txt
+++ b/requirements/REQUIREMENTS-NAPARI-CI.txt
@@ -142,7 +142,7 @@ superqt==0.7.6
 sympy==1.14.0
 tabulate==0.9.0
 threadpoolctl==3.6.0
-tifffile==2024.8.30
+tifffile==2025.10.16
 tomli==2.3.0
 tomli_w==1.2.0
 toolz==1.1.0
diff --git a/starfish/REQUIREMENTS-STRICT.txt b/starfish/REQUIREMENTS-STRICT.txt
index 864dc021..9f23012c 100644
--- a/starfish/REQUIREMENTS-STRICT.txt
+++ b/starfish/REQUIREMENTS-STRICT.txt
@@ -49,7 +49,7 @@ six==1.17.0
 slicedimage==4.1.1
 sympy==1.14.0
 threadpoolctl==3.6.0
-tifffile==2024.8.30
+tifffile==2025.10.16
 tqdm==4.67.1
 trackpy==0.7
 typing_extensions==4.15.0