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Copy pathProcess4D_TrackDisk.m
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Process4D_TrackDisk.m
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%% Read Empad Data
data_dir = 'data/';
wdir = 'data/';
%wdir = 'C:/Users/in931/Desktop/';
%fname = 'tas2microprobe_06_1150kx_cl1p5m_ap50_0p44mrad_spot8_mono60_80kV_93K_50x_50y_100z_432step_x128_y128.raw';
fname = '05_TaS2_250C_80keV_450kx_CL1p9m_10um_0_2mrad_spot6_shiftdiff_50x_50y_100z_432step_x128_y128.raw';
fname = '11_TaS2_250C_80keV_80kx_CL1p9m_10um_0_2mrad_spot6_shiftdiff_50x_50y_100z_432step_x128_y128.raw';
%fname = '12_TaS2_250C_80keV_450kx_CL1p9m_10um_0_2mrad_spot6_shiftdiff_50x_50y_100z_864step_x64_y64.raw';
e = empad( [wdir,fname],128);
%e2 = empad( [wdir, fname], 64 );
vis4D(e2.im4D)
%% Get Average CBED;
CBED_ave = e.pacbed;
%% Manually choose disk positions
imageBC(CBED_ave)
title('Adjust Contrast and Hit Apply, then Return')
pause
[x0, y0] = em_clickAddRemove([], [], CBED_ave, false);
x0 = round(x0);
y0 = round(y0);
%% Fit Gaussian to averaged CBED to estimate parameters
numDisk = length(x0);
padding = 4;
nsx = e.nsx;
nsy = e.nsy;
centers = zeros(numDisk,nsx,nsy,2);
radii = zeros(numDisk,nsx,nsy,1);
for disk_ind = 1:numDisk
[indep(:,:,1), indep(:,:,2)] = meshgrid(1:2*padding+1,1:2*padding+1);
subIm = CBED_ave( y0(disk_ind)-padding:y0(disk_ind)+padding,x0(disk_ind)-padding:x0(disk_ind)+padding);
param_guess = [padding+1, padding+1, padding, max(subIm(:)), min(subIm(:))];
lb = [padding/2, padding/2, 0, 0, 0];
ub = [3*padding/2, 3*padding/2, inf,inf,inf];
tol = 1e-7;
maxFunEvals = 1e6;
maxIter = 1e3;
opt = optimset('Display','Iter','TolFun',tol,'TolX',tol,'MaxFunEvals',maxFunEvals,'MaxIter',maxIter);
param_fit = lsqcurvefit(@gaus2d, param_guess, indep, subIm,lb,ub,opt);
x0(disk_ind) = x0(disk_ind)+round( param_fit(1) )-padding-1 ;
y0(disk_ind) = y0(disk_ind)+round( param_fit(2) )-padding-1 ;
subIm_cor = CBED_ave( y0(disk_ind)-padding:y0(disk_ind)+padding,x0(disk_ind)-padding:x0(disk_ind)+padding);
figure
subplot(1,3,1)
imagesc(subIm);
subplot(1,3,2)
imagesc(gaus2d(param_fit,indep));
subplot(1,3,3)
imagesc(subIm_cor)
%%
% m = median(subIm(:));
% s = std(subIm(:));
% param_guess = param_fit;
% lb = [param_guess(1)-2, param_guess(2)-2, param_guess(3)*0.8, 0,0];
% ub = [param_guess(1)+2, param_guess(2)+2, param_guess(3)*1.2, inf,inf];
%
% progress= zeros(nsy,nsx);
%
% % %
% subplot(1,3,1)
% h_subim = imagesc(progress);
% axis equal off
% subplot(1,3,2)
% h_fit = imagesc(progress);
% axis equal off
% subplot(1,3,3)
% h_progress = imagesc(progress);
% axis equal off
% drawnow
for sx = 1:nsx
disp(sx)
for sy = 1:nsy
for disk_ind = 1:numDisk
% Get Sub Matrix
cur_subIm = squeeze(e.im4D( y0(disk_ind)-padding:y0(disk_ind)+padding,x0(disk_ind)-padding:x0(disk_ind)+padding,sx,sy));
[xc, yc] = centerOfMass(cur_subIm);
centers(disk_ind,sx,sy,:) = [xc,yc] + [x0(disk_ind)-padding-1, y0(disk_ind)-padding-1];
% remove outlier of image
% cur_subIm( cur_subIm < m -s) = m-s;
% cur_subIm( cur_subIm > m +s) = m+s;
%
% % Fit Gaussian
% cur_param_fit = lsqcurvefit(@gaus2d, param_guess, indep, subIm,lb,ub,opt);
%
% Save center and sigma
% centers(disk_ind,sx,sy,:) = cur_param_fit(1:2) + [x0(disk_ind)-padding-1, y0(disk_ind)-padding-1];
% radii(disk_ind,sx,sy) = cur_param_fit(3);
% set(h_subim, 'CData', cur_subIm);
% set(h_fit, 'CData', gaus2d(cur_param_fit,indep));
% drawnow;
end
end
% progress(:,sx) = ones(nsy,1);
% set(h_progress, 'CData', progress);
% drawnow
disp(sx)
end
pause
end
%% Analysis
% Choose reference point
dev = centers - centers(:,1,1,:);
dr = sqrt( dev(:,:,:,1).^2 + dev(:,:,:,2).^2);
figure
for d = 1: 12
subplot(3,4,d)
imagesc(squeeze(dr(d,:,:,:)))
axis equal off
colormap('inferno')
end