diff --git a/CHANGELOG.md b/CHANGELOG.md
index 3f7f160d899f..d76c07b34352 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 ### Fixed
 
 - Ensure `color-mix(…)` polyfills do not cause used CSS variables to be removed ([#17555](https://github.com/tailwindlabs/tailwindcss/pull/17555))
+- Ensure the `color-mix(…)` polyfill creates fallbacks for theme variables that reference other theme variables ([#17562](https://github.com/tailwindlabs/tailwindcss/pull/17562))
 
 ## [4.1.3] - 2025-04-04
 
diff --git a/packages/tailwindcss/src/ast.ts b/packages/tailwindcss/src/ast.ts
index f15191118d68..3db0432dd3a9 100644
--- a/packages/tailwindcss/src/ast.ts
+++ b/packages/tailwindcss/src/ast.ts
@@ -542,6 +542,7 @@ export function optimizeAst(
         let requiresPolyfill = false
         ValueParser.walk(ast, (node, { replaceWith }) => {
           if (node.kind !== 'function' || node.value !== 'color-mix') return
+
           let containsUnresolvableVars = false
           let containsCurrentcolor = false
           ValueParser.walk(node.nodes, (node, { replaceWith }) => {
@@ -550,17 +551,47 @@ export function optimizeAst(
               requiresPolyfill = true
               return
             }
-            if (node.kind !== 'function' || node.value !== 'var') return
-            let firstChild = node.nodes[0]
-            if (!firstChild || firstChild.kind !== 'word') return
-            requiresPolyfill = true
-            let inlinedColor = designSystem.theme.resolveValue(null, [firstChild.value as any])
-            if (!inlinedColor) {
-              containsUnresolvableVars = true
-              return
-            }
+
+            let varNode: ValueParser.ValueAstNode | null = node
+            let inlinedColor: string | null = null
+            let seenVariables = new Set<string>()
+            do {
+              if (varNode.kind !== 'function' || varNode.value !== 'var') return
+              let firstChild = varNode.nodes[0]
+              if (!firstChild || firstChild.kind !== 'word') return
+
+              let variableName = firstChild.value
+
+              if (seenVariables.has(variableName)) {
+                containsUnresolvableVars = true
+                return
+              }
+
+              seenVariables.add(variableName)
+
+              requiresPolyfill = true
+
+              inlinedColor = designSystem.theme.resolveValue(null, [firstChild.value as any])
+              if (!inlinedColor) {
+                containsUnresolvableVars = true
+                return
+              }
+              if (inlinedColor.toLowerCase() === 'currentcolor') {
+                containsCurrentcolor = true
+                return
+              }
+
+              if (inlinedColor.startsWith('var(')) {
+                let subAst = ValueParser.parse(inlinedColor)
+                varNode = subAst[0]
+              } else {
+                varNode = null
+              }
+            } while (varNode)
+
             replaceWith({ kind: 'word', value: inlinedColor })
           })
+
           if (containsUnresolvableVars || containsCurrentcolor) {
             let separatorIndex = node.nodes.findIndex(
               (node) => node.kind === 'separator' && node.value.trim().includes(','),
diff --git a/packages/tailwindcss/src/index.test.ts b/packages/tailwindcss/src/index.test.ts
index 707e8f02c875..411e3fe7b426 100644
--- a/packages/tailwindcss/src/index.test.ts
+++ b/packages/tailwindcss/src/index.test.ts
@@ -4764,6 +4764,36 @@ describe('`color-mix(…)` polyfill', () => {
     `)
   })
 
+  it('creates an inlined variable version of the color-mix(…) usages when it resolves to a var(…) containing another theme variable', async () => {
+    await expect(
+      compileCss(
+        css`
+          @theme {
+            --color-red: var(--color-red-500);
+            --color-red-500: oklch(63.7% 0.237 25.331);
+          }
+          @tailwind utilities;
+        `,
+        ['text-red/50'],
+      ),
+    ).resolves.toMatchInlineSnapshot(`
+      ":root, :host {
+        --color-red: var(--color-red-500);
+        --color-red-500: oklch(63.7% .237 25.331);
+      }
+
+      .text-red\\/50 {
+        color: #fb2c3680;
+      }
+
+      @supports (color: color-mix(in lab, red, red)) {
+        .text-red\\/50 {
+          color: color-mix(in oklab, var(--color-red) 50%, transparent);
+        }
+      }"
+    `)
+  })
+
   it('works for color values in the first and second position', async () => {
     await expect(
       compileCss(
@@ -4971,6 +5001,34 @@ describe('`color-mix(…)` polyfill', () => {
     `)
   })
 
+  it('uses the first color value as the fallback when the `color-mix(…)` function contains theme variables that resolves to other variables', async () => {
+    await expect(
+      compileCss(
+        css`
+          @tailwind utilities;
+          @theme {
+            --color-red: var(--my-red);
+          }
+        `,
+        ['text-red/50'],
+      ),
+    ).resolves.toMatchInlineSnapshot(`
+      ".text-red\\/50 {
+        color: var(--color-red);
+      }
+
+      @supports (color: color-mix(in lab, red, red)) {
+        .text-red\\/50 {
+          color: color-mix(in oklab, var(--color-red) 50%, transparent);
+        }
+      }
+
+      :root, :host {
+        --color-red: var(--my-red);
+      }"
+    `)
+  })
+
   it('uses the first color value of the inner most `color-mix(…)` function as the fallback when nested `color-mix(…)` function all contain non-theme variables', async () => {
     await expect(
       compileCss(