-
Notifications
You must be signed in to change notification settings - Fork 8
Description
Hi,
I got below errors in an alignment step by mafft.
I suspect that it is caused by asterisks in vsearch output.
Do you have any solutions to this problems?
--
[2020-08-04 13:52:17] Generated nanoCLUSTdef/p5.uc
Removing nanoCLUSTdef/p5_u.fasta
Removing nanoCLUSTdef/p5_n.fasta
Removing nanoCLUSTdef/p5_s.fasta
Removing nanoCLUSTdef/p5_r.fasta
nanoCLUSTdef/p1.uc
nanoCLUSTdef/p2.uc
nanoCLUSTdef/p3.uc
nanoCLUSTdef/p4.uc
nanoCLUSTdef/p5.uc
[2020-08-04 13:52:18] Generating nanoCLUSTdef/.fasta
Taking at most 50 sequences at 10% of average bin size (1308.70035971,1454.11151079,1599.52266187)
Using 50 sequences
[2020-08-04 13:52:18] Aligning nanoCLUSTdef/.fasta
/usr/local/bin/mafft-ginsi nanoCLUSTdef/.fasta > nanoCLUSTdef/.gfasta
[2020-08-04 13:52:18] Generated nanoCLUSTdef/*.gfasta
Traceback (most recent call last):
File "./nanopore/nanoCLUST.py", line 407, in
main(sys.argv[1:])
File "./nanopore/nanoCLUST.py", line 385, in main
alignments=AlignIO.read(output_folder+"/"+i+".gfasta","fasta")
File "/Users/home/anaconda3/envs/python2/lib/python2.7/site-packages/Bio/AlignIO/init.py", line 433, in read
raise ValueError("No records found in handle")
ValueError: No records found in handle