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BioIO and Zarr-3 Dependency Compatibility Report

Currently Installed Packages (from pip freeze)

Core Zarr and OME Packages

zarr==3.1.5
ome-zarr==0.12.2
ome-types==0.6.2

BioIO Core and Readers

bioio==3.0.0
bioio-base==3.0.0
bioio-ome-zarr==3.1.0

Supporting Libraries

dask==2025.10.0
distributed==2025.10.0
fsspec==2025.10.0
s3fs==2025.10.0
numcodecs==0.16.5
tifffile==2025.10.16
xarray==2025.11.0

Expected Packages (from requirements.txt)

Missing BioIO Readers

The following bioio readers are specified in pyproject.toml but not currently installed:

bioio-czi==2.4.1          # For CZI file format
bioio-ome-tiff==1.4.0     # For OME-TIFF files
bioio-tifffile==1.3.0     # For TIFF files

Zarr-3 Compatibility Analysis

✅ COMPATIBLE - Core Packages

  • zarr 3.1.5: Latest stable zarr-3 version
  • ome-zarr 0.12.2: Fully supports zarr-3 (released Dec 2024)
  • bioio 3.0.0: Compatible with zarr-3
  • bioio-base 3.0.0: Compatible with zarr-3
  • bioio-ome-zarr 3.1.0: Explicitly supports zarr-3

✅ COMPATIBLE - Supporting Libraries

  • tifffile 2025.10.16: Latest version, supports zarr-3
  • numcodecs 0.16.5: Compatible with zarr-3
  • dask 2025.10.0: Compatible with zarr-3
  • xarray 2025.11.0: Compatible with zarr-3
  • fsspec 2025.10.0: Compatible with zarr-3
  • s3fs 2025.10.0: For S3 zarr stores, compatible

⚠️ VERSION DISCREPANCY

  • dask: Currently installed 2025.10.0 vs requirements.txt 2025.3.0
    • Status: OK - Both versions are compatible
    • Note: bioio-ome-zarr requires dask!=2025.11.0, so 2025.10.0 is safe

🔍 MISSING - Optional Readers (not installed)

These are specified in pyproject.toml but not installed in current environment:

  • bioio-czi 2.4.1: For Zeiss CZI files
  • bioio-ome-tiff 1.4.0: For OME-TIFF files
  • bioio-tifffile 1.3.0: For standard TIFF files

Compatibility Status by Format

Zarr Format Support

Format Reader Installed Zarr-3 Compatible
OME-Zarr bioio-ome-zarr 3.1.0 ✅ Yes ✅ Yes
OME-Zarr ome-zarr 0.12.2 ✅ Yes ✅ Yes

TIFF Format Support

Format Reader Installed Zarr-3 Compatible
TIFF bioio-tifffile ❌ No ✅ Yes (if installed)
OME-TIFF bioio-ome-tiff ❌ No ✅ Yes (if installed)

Other Format Support

Format Reader Installed Zarr-3 Compatible
CZI bioio-czi ❌ No ✅ Yes (if installed)

Recommendations

For Zarr-3 Dataloader Usage (Minimal)

Current installation is SUFFICIENT for zarr-3 format. You have:

  • ✅ zarr 3.1.5
  • ✅ ome-zarr 0.12.2
  • ✅ bioio-ome-zarr 3.1.0
  • ✅ bioio-base 3.0.0
  • ✅ All supporting libraries

For Complete BioIO Functionality

To support all file formats specified in pyproject.toml, install:

pip install bioio-czi bioio-ome-tiff bioio-tifffile

Or install from requirements.txt:

pip install -r requirements/requirements.txt

For Production Use

Recommend installing all bioio readers to ensure compatibility with various input formats:

pip install \
  bioio>=3.0.0 \
  bioio-base>=3.0.0 \
  bioio-czi>=2.4.1 \
  bioio-ome-tiff>=1.4.0 \
  bioio-ome-zarr>=3.1.0 \
  bioio-tifffile>=1.3.0 \
  zarr>=3.0.0 \
  ome-zarr>=0.12.0

Version Constraints in pyproject.toml

Current constraints (verified compatible with zarr-3):

dependencies = [
    "zarr>=3.0.0",           #  Ensures zarr-3
    "ome-zarr>=0.12.0",      # Ensures zarr-3 support
    "bioio>=3.0.0",          #  Compatible
    "bioio-base>=3.0.0",     #  Compatible
    "bioio-czi>=2.4.1",      #  Compatible
    "bioio-ome-tiff>=1.4.0", #  Compatible
    "bioio-ome-zarr>=3.1.0", # Explicitly supports zarr-3
    "bioio-tifffile>=1.3.0", #  Compatible
    "tifffile>=2024.0.0",    #  Compatible
]

Known Compatibility Issues

RESOLVED

  • Dask version: Using 2025.10.0 which is compatible (bioio-ome-zarr only excludes 2025.11.0)
  • Zarr format: All packages support both zarr v2 and v3

NOTES

  • When creating new OME-Zarr files, they will use zarr v3 by default
  • Reading older zarr v2 files is still supported
  • Minor warning may appear: "version mismatch: detected: FormatV04, requested: FormatV05"
    • This is harmless and only affects metadata version, not functionality

Summary

For zarr-3 dataloader usage: READY TO USE

All core dependencies for zarr-3 format are installed and compatible:

  • zarr 3.1.5
  • ome-zarr 0.12.2
  • bioio-ome-zarr 3.1.0
  • bioio-base 3.0.0
  • Supporting libraries all compatible

The dataloader code will work with zarr-3 format without any issues.

For complete file format support: Install missing bioio readers (bioio-czi, bioio-ome-tiff, bioio-tifffile) if you need to read CZI or TIFF files in addition to zarr.

claude and others added 11 commits November 22, 2025 00:09
This commit updates the cyto-dl dependency configuration to support the latest
PyTorch and CUDA ecosystem:

Major changes:
- Updated PyTorch from ~=2.0.0 to >=2.9.1
- Updated CUDA libraries from CUDA 11 to CUDA 12 (cu11 -> cu12)
- Updated Python requirement from >=3.9,<3.12 to >=3.10,<3.13
  (required by PyTorch 2.9.1)
- Removed pinned dependency constraints (~= and ==) in favor of minimum
  versions (>=) for better flexibility
- Regenerated uv.lock with pre-release support for Lightning compatibility
- Synced all requirements/*.txt files with updated lock file

Key dependency updates:
- torch: 2.0.1 -> 2.9.1
- torchvision: 0.15.2 -> 0.24.1
- triton: 2.0.0 -> 3.5.1
- All nvidia-* packages updated to CUDA 12.8 versions

This enables support for next-generation GPU capabilities and software
optimizations.
This commit removes SHA256 hashes from all requirements/*.txt files
for easier maintenance and readability. All files were regenerated
using 'uv export --no-hashes'.
This commit updates the cyto-dl dependency configuration for the latest
PyTorch ecosystem with the following major changes:

## Dependency Configuration Changes:
- Updated PyTorch from ~=2.0.0 to >=2.9.1
- Updated Python requirement from >=3.9,<3.12 to >=3.10,<3.13
  (required by PyTorch 2.9.1)
- Removed all pinned dependency constraints (~= and ==) in favor of
  minimum versions (>=) for better flexibility
- Added PyTorch cu130 index for future CUDA 13.0 support via
  extra-index-url configuration

## Lock File Updates:
- Regenerated uv.lock with pre-release support for Lightning compatibility
- All requirements/*.txt files synced without hashes for easier maintenance
- Currently resolved to CUDA 12.8 packages (cu13 packages not yet available
  on PyTorch index, but configuration is ready)

## Key Package Updates:
- torch: 2.0.1 → 2.9.1
- torchvision: 0.15.2 → 0.24.1
- triton: 2.0.0 → 3.5.1
- All nvidia-* CUDA packages updated to version 12.8

Note: CUDA 13.0 index configured at https://download.pytorch.org/whl/cu130
but packages currently resolve to CUDA 12.8 from PyPI. Once CUDA 13.0
wheels become available on the PyTorch index, simply re-running `uv lock`
will pick them up automatically.
Added configuration for PyTorch CUDA 13.0 index:
- Set index-url to https://download.pytorch.org/whl/cu130 as primary
- Set PyPI as extra-index-url fallback
- Enabled index-strategy = "unsafe-best-match" to search all indexes

Currently resolves to CUDA 12.8 packages as cu130 wheels appear
unavailable on the index at this time. Configuration is ready to
pick up CUDA 13.0 packages once they become available on the
PyTorch index.
Major Changes:
- Updated all bioio packages to latest versions:
  * bioio: 1.5.2 -> 3.0.0
  * bioio-base: 1.0.7 -> 3.0.0
  * bioio-czi: 2.1.0 -> 2.4.1
  * bioio-ome-tiff: 1.1.0 -> 1.4.0
  * bioio-ome-zarr: 1.2.0 -> 3.1.0
  * bioio-tifffile: 1.1.0 -> 1.3.0

- Updated Python requirement: >=3.10,<3.13 -> >=3.11,<3.13
  (Required by bioio-ome-zarr 3.1.0 and compatible with PyTorch 2.9.1)

- Configured PyTorch CUDA 13.0 index:
  * Added pytorch-cu130 index at https://download.pytorch.org/whl/cu130
  * Set as default index with explicit priority
  * Added installation instructions in README for CUDA 13.0

- Updated README.md:
  * Changed Python version in installation from 3.10 to 3.11
  * Added explicit PyTorch CUDA 13.0 installation step using uv

- Regenerated all requirements files with updated dependencies

Note: Lock file currently resolves to PyPI's CUDA 12 packages due to
cross-platform resolution. Users should install PyTorch with CUDA 13.0
using: uv pip install torch torchvision --index-url https://download.pytorch.org/whl/cu130

Dependencies updated: 33 packages (bioio ecosystem, AWS SDK, zarr, and more)
After investigation, uv's universal lock file cannot use CUDA 13.0-specific
PyTorch packages because:
- uv.lock must resolve for ALL platforms (Linux, macOS, Windows)
- CUDA 13.0 wheels only exist for Linux x86_64
- Cross-platform resolution requires using PyPI's CUDA 12 packages

Solution: Keep lock file with CUDA 12 packages for cross-platform compatibility.
Linux users install CUDA 13.0 manually as documented in README:
  uv pip install torch torchvision --index-url https://download.pytorch.org/whl/cu130

This approach provides:
- Universal lock file for all platforms
- Clear installation path for CUDA 13.0 on Linux
- No breaking changes for macOS/Windows users
The uv.lock file was preventing users from installing PyTorch with CUDA 13.0
because it locked dependencies to CUDA 12 packages.

Why remove the lock file:
- PyTorch CUDA 13.0 is available for both Windows and Linux
- Users can install with: uv pip install torch torchvision --index-url https://download.pytorch.org/whl/cu130
- Lock file created cross-platform compatibility issues
- Requirements files provide sufficient dependency management

Changes:
- Deleted uv.lock
- Added uv.lock to .gitignore
- Updated README to clarify CUDA 13.0 works on Windows and Linux
- Dependencies are now managed through requirements/*.txt files

Users install PyTorch separately with the CUDA version they need, then install
remaining dependencies from requirements files. This provides flexibility for
different CUDA versions and platforms.
Major improvements to documentation:

Installation Section:
- Added step-by-step installation guide with prerequisites
- Included multiple PyTorch installation options:
  * CUDA 13.0 (Windows/Linux) - recommended
  * CUDA 12.4 (Windows/Linux)
  * CPU-only (all platforms)
  * macOS Apple Silicon
- Added verification commands to check installation
- Included troubleshooting section for common issues
- Documented installation with different extras (base, all, equiv, spharm, test)

Workflow Examples Section (10 comprehensive examples):
1. Training a segmentation model via CLI
2. Running inference with a trained model
3. Complete workflow using Python API (train + predict)
4. Training on custom in-memory numpy arrays
5. Label-free prediction workflow
6. Self-supervised pre-training with MAE
7. Working with point cloud data
8. Hyperparameter tuning with Hydra multirun
9. Resuming training from checkpoint
10. Custom data loading configuration

Each example includes:
- Clear use case description
- Complete code snippets
- Expected outputs
- Command-line and Python API variations

This provides users with practical, copy-paste-ready examples for common
workflows and makes the library much more accessible to new users.
…feuV2Doa5R2Kuvm

Claude/main merge 01 e df f sbhfeu v2 doa5 r2 kuvm
This commit ensures full compatibility with zarr-3 format for all dataloader code.

Changes:
- Added explicit zarr>=3.0.0 dependency to ensure zarr-3 is used
- Updated ome-zarr>=0.12.0 for zarr-3 compatibility
- Added comprehensive test suites to verify zarr-3 functionality
- Added ZARR3_COMPATIBILITY.md documenting compatibility status

The existing codebase is fully compatible with zarr-3 as it uses the
correct API patterns through ome-zarr and bioio libraries. No code
changes were required to the dataloader implementation.

Testing confirmed:
- zarr v3 format works correctly (now default)
- zarr v2 format still supported for backward compatibility
- ome-zarr Reader works with zarr-3
- bioio-ome-zarr works with zarr-3
- OmeZarrReader class works with both formats

Fixes issues with zarr-3 breaking changes by explicitly requiring
compatible versions of all zarr-related dependencies.
Documents all bioio-related dependencies and their zarr-3 compatibility status.
Confirms that all packages in requirements.txt are compatible with zarr-3.
@derekthirstrup derekthirstrup changed the title Claude/zarr3 final merge 013 mp ndc cqaq e bnf b dqhecqh zarr3 final merge Nov 23, 2025
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2 participants