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10 changes: 5 additions & 5 deletions README.md
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# biotinIDR : Contains code and input files for Biotin IDR study
### Authors : David Paul Minde and Manasa Ramakrishna

#### Main code : R and Python code for analysing data

### Main code : R and Python code for analysing data
Note: Before you run this code, you will need to download the following scripts
1. R installed on your computer
2. Python installed on your computer
Expand All @@ -23,7 +21,7 @@ d2p2.py | Tom's code to use the d2p2 API to use 9 different callers to identify
protinfo.py | Tom's code to query uniprot/swissprot database on Ensembl | Python


#### Input : Folder containing all input files needed for code to run
### Input : Folder containing all input files needed for code to run

File| Contents
----------|-------------
Expand All @@ -38,6 +36,7 @@ HEK293-with-IDR-VSL2b-and-PTMs.rds | HEK293 peoteins from Geiger et al with PTMs
PTMs-by-gene-from-Phosphositeplus.rds | PTM data from PhosphositePlus summarised by gene and for phosphorylation, ubiquitination, sumoylation, acetylation
go-pro-kegg.rda | An object that contains all GO, Interpro and KEGG annotations for proteins in each of the 4 studies


#### RDS files can be read using the command readRDS as shown below
```
readRDS("Input/Biotin-PTM-IDR-data-for-all-studies-and-callers.rds")
Expand All @@ -48,7 +47,7 @@ readRDS("Input/Biotin-PTM-IDR-data-for-all-studies-and-callers.rds")
load("Input/go-pro-kegg.rda")
```

#### Output : A description of all files generated by running nods_final.Rmd.
### Output : A description of all files generated by running nods_final.Rmd.

Date_Output : Date-tagged output folder This folder should contain the following files
Date = Date of the analysis in format yyyy-mm-dd ; Time = Time of analysis in format hh:mm:ss
Expand Down Expand Up @@ -82,6 +81,7 @@ Date_Time_Effect-size-for-pairwise-comparison-of-IDR-classes-and-parameters-VSL2
Date_Time_VSL2b_Pairwise-t-test-pvals.txt
Date_Time_VSL2b_Tukey-HSD-ci-pvals.txt

### Figures : Folder containing all main and supplementary Figures from the paper

#### SupplTables.xlsx :
Supplementary tables referred to in the manuscript. Contain data that can be queried/used. Contains 5 datasheets and an "Index" sheet describing each of the data sheets.

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