Hello, I hope you're well. Lately, when I have been using the premessa Debarcoder, the cell counts I get on the y-axis of the bar chart and in the separation curve has been in the 1000s, but each debarcoded sample actually has cell/event counts in 100,000s (which I can see when i upload the debarcoded FCS file into FlowJo). I've attached some screenshots to demonstrate.
When I started using the debarcoder many months ago, it didn't seem to have this problem previously. I'm not sure if this is affecting the actual debarcoding of the sample.
Thanks in advance for your help/reply.
Debarcoder screenshot with cell counts.pdf
Debarcoder separation screenshot with cell counts.pdf
FlowJo screenshot with event counts.pdf
Best,
Rebecca
Hello, I hope you're well. Lately, when I have been using the premessa Debarcoder, the cell counts I get on the y-axis of the bar chart and in the separation curve has been in the 1000s, but each debarcoded sample actually has cell/event counts in 100,000s (which I can see when i upload the debarcoded FCS file into FlowJo). I've attached some screenshots to demonstrate.
When I started using the debarcoder many months ago, it didn't seem to have this problem previously. I'm not sure if this is affecting the actual debarcoding of the sample.
Thanks in advance for your help/reply.
Debarcoder screenshot with cell counts.pdf
Debarcoder separation screenshot with cell counts.pdf
FlowJo screenshot with event counts.pdf
Best,
Rebecca