-
Notifications
You must be signed in to change notification settings - Fork 237
Auto-estimate margins based on freq_min/f0+Q for filters #4227
New issue
Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.
By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.
Already on GitHub? Sign in to your account
Merged
Merged
Changes from 7 commits
Commits
Show all changes
21 commits
Select commit
Hold shift + click to select a range
f66ea6a
auto-estimate margins based on freq_min/f0+Q for filters
alejoe91 7e78464
NotchFilter uses FilterRecording
alejoe91 b846118
Notch filter is 'ba'
alejoe91 18f270a
Merge branch 'main' into auto-fix-margin
alejoe91 4149305
Better margin behavior and 'Extract LFPs' tutorial
alejoe91 d08c378
Add new how-to to index
alejoe91 79e4469
Fix notch
alejoe91 c1e80f3
Move how-to in gallery and fox ibl export tests
alejoe91 a8df357
Remove pre-generated how-to files for extract lfp
alejoe91 7e89bee
Clean up forhowto files and add to gitignore
alejoe91 3de9a15
More info on forhowto
alejoe91 76cfcc4
rst needed to be rst...
alejoe91 a0d243f
rst needed to be rst...
alejoe91 6c0dd0f
Add seaborn to docs requirements
alejoe91 f0fdd34
Make recording smaller
alejoe91 f1f47bb
cache to folder instead of memory
alejoe91 22bc2d7
Add ignore_low_freq_error to kwargs
alejoe91 d561025
Chris' comments
alejoe91 5d80462
Merge branch 'main' of github.com:SpikeInterface/spikeinterface into …
alejoe91 b70b2fa
Make margin_ms None by default in BaseFilterRecording
alejoe91 9675c31
Fix tests
alejoe91 File filter
Filter by extension
Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
There are no files selected for viewing
Large diffs are not rendered by default.
Oops, something went wrong.
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
This file contains hidden or bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
This file contains hidden or bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
| Original file line number | Diff line number | Diff line change |
|---|---|---|
| @@ -0,0 +1,310 @@ | ||
| # --- | ||
| # jupyter: | ||
| # jupytext: | ||
| # cell_metadata_filter: -all | ||
| # formats: ipynb,py:percent | ||
| # text_representation: | ||
| # extension: .py | ||
| # format_name: percent | ||
| # format_version: '1.3' | ||
| # jupytext_version: 1.18.1 | ||
| # kernelspec: | ||
| # display_name: Python 3 (ipykernel) | ||
| # language: python | ||
| # name: python3 | ||
| # --- | ||
|
|
||
| # %% [markdown] | ||
| # # Extract LFPs | ||
| # | ||
| # ### Understanding filtering artifacts and chunking when extracting LFPs | ||
| # | ||
| # Local Field Potentials (LFPs) are low-frequency signals (<300 Hz) that reflect the summed activity of many neurons. | ||
| # Extracting LFPs from high-sampling-rate recordings requires bandpass filtering, but this can introduce artifacts | ||
| # when not done carefully, especially when data is processed in chunks (for memory efficiency). | ||
| # | ||
| # This tutorial demonstrates: | ||
| # 1. How to generate simulated LFP data | ||
| # 2. Common pitfalls when filtering with low cutoff frequencies | ||
| # 3. How chunking and margins affect filtering artifacts | ||
| # 4. Summary | ||
| # | ||
| # **Key takeaway**: For LFP extraction, use large chunks (30-60s) and large margins (several seconds) to minimize | ||
| # edge artifacts, even though this is less memory-efficient. | ||
|
|
||
| # %% | ||
| import time | ||
| import numpy as np | ||
| import matplotlib.pyplot as plt | ||
| from pathlib import Path | ||
| import pandas as pd | ||
| import seaborn as sns | ||
|
|
||
| import spikeinterface as si | ||
| import spikeinterface.extractors as se | ||
| import spikeinterface.preprocessing as spre | ||
| import spikeinterface.widgets as sw | ||
| from spikeinterface.core import generate_ground_truth_recording | ||
|
|
||
| # %% | ||
| # %matplotlib inline | ||
|
|
||
| # %% [markdown] | ||
| # ## 1. Generate simulated recording with low-frequency signals | ||
| # | ||
| # Let's create a simulated recording and add some low-frequency sinusoids that mimic LFP activity. | ||
|
|
||
| # %% | ||
| # Generate a ground truth recording with spikes | ||
| # Use a higher sampling rate (30 kHz) to simulate raw neural data | ||
| recording, sorting = generate_ground_truth_recording( | ||
| durations=[300.0], # 300 s | ||
| sampling_frequency=30000.0, | ||
| num_channels=1, | ||
| num_units=4, | ||
| seed=2305, | ||
| ) | ||
|
|
||
| print(f"Recording: {recording}") | ||
| print(f"Duration: {recording.get_total_duration():.1f} s") | ||
| print(f"Sampling frequency: {recording.sampling_frequency} Hz") | ||
| print(f"Number of channels: {recording.get_num_channels()}") | ||
|
|
||
| # %% [markdown] | ||
| # Now let's add some low-frequency sinusoidal components to simulate LFP signals | ||
|
|
||
| # %% | ||
| # Add low-frequency sinusoids with different frequencies and phases per channel | ||
| np.random.seed(2305) | ||
| num_channels = recording.get_num_channels() | ||
| lfp_signals = np.zeros((recording.get_num_samples(), recording.get_num_channels())) | ||
| time_vector = recording.get_times() | ||
|
|
||
| for ch in range(num_channels): | ||
| # Add multiple frequency components (theta, alpha, beta ranges) | ||
| # Theta-like: 4-8 Hz | ||
| freq_theta = 4 + np.random.rand() * 4 | ||
| phase_theta = np.random.rand() * 2 * np.pi | ||
| amp_theta = 50 + np.random.rand() * 50 | ||
|
|
||
| # Alpha-like: 8-12 Hz | ||
| freq_alpha = 8 + np.random.rand() * 4 | ||
| phase_alpha = np.random.rand() * 2 * np.pi | ||
| amp_alpha = 30 + np.random.rand() * 30 | ||
|
|
||
| # Beta-like: 12-30 Hz | ||
| freq_beta = 12 + np.random.rand() * 18 | ||
| phase_beta = np.random.rand() * 2 * np.pi | ||
| amp_beta = 20 + np.random.rand() * 20 | ||
|
|
||
| lfp_signals[:, ch] = ( | ||
| amp_theta * np.sin(2 * np.pi * freq_theta * time_vector + phase_theta) + | ||
| amp_alpha * np.sin(2 * np.pi * freq_alpha * time_vector + phase_alpha) + | ||
| amp_beta * np.sin(2 * np.pi * freq_beta * time_vector + phase_beta) | ||
| ) | ||
|
|
||
| # Create a recording with the added LFP signals | ||
| recording_lfp = si.NumpyRecording(traces_list=[lfp_signals], sampling_frequency=recording.sampling_frequency, | ||
| channel_ids=recording.channel_ids) | ||
| recording_with_lfp = recording + recording_lfp | ||
|
|
||
| print("Added low-frequency components to simulate LFP signals") | ||
|
|
||
| # %% [markdown] | ||
| # Let's visualize a short segment of the signal | ||
|
|
||
| # %% | ||
| sw.plot_traces(recording_with_lfp, time_range=[0, 3]) | ||
|
|
||
| # %% [markdown] | ||
| # ## 2. Filtering with low cutoff frequencies: the problem | ||
| # | ||
| # Now let's try to extract LFPs using a bandpass filter with a low highpass cutoff (1 Hz). | ||
| # This will demonstrate a common issue. | ||
|
|
||
| # %% | ||
| # Try to filter with 1 Hz highpass | ||
| try: | ||
| recording_lfp_1hz = spre.bandpass_filter(recording_with_lfp, freq_min=1.0, freq_max=300.0) | ||
| print("Filtering succeeded!") | ||
| except Exception as e: | ||
| print(f"Error message:\n{str(e)}") | ||
|
|
||
| # %% [markdown] | ||
| # **Why does this fail?** | ||
| # | ||
| # The error occurs because by default in SpikeInterface when highpass filtering below 100 Hz. | ||
| # Filters with very low cutoff frequencies have long impulse responses, which require larger margins to avoid edge artifacts between chunks. | ||
| # | ||
| # The filter length (and required margin) scales inversely with the highpass frequency. A 1 Hz highpass | ||
| # filter requires a margin of several seconds, while a 300 Hz highpass (for spike extraction) only needs | ||
| # a few milliseconds. | ||
|
|
||
| # %% [markdown] | ||
| # ## 3. Understanding chunking and margins | ||
| # | ||
| # SpikeInterface processes recordings in chunks to handle large datasets efficiently. Each chunk needs | ||
| # a "margin" (extra samples at the edges) to avoid edge artifacts when filtering. Let's demonstrate | ||
| # this by saving the filtered data with different chunking strategies. | ||
| # | ||
| # **This error is to inform the user that extra care should be used when dealing with LFP signals!** | ||
| # | ||
| # We can ignore this error, but let's make sure we understand what it's happening. | ||
|
|
||
| # %% | ||
| # We can ignore this error, but let's see what is happening | ||
| recording_filt = spre.bandpass_filter(recording_with_lfp, freq_min=1.0, freq_max=300.0, ignore_low_freq_error=True) | ||
|
|
||
| # %% [markdown] | ||
| # When retrieving traces, extra samples will be retrieved at the left and right edges. | ||
| # By default, the filter function will set a margin to 5x the sampling period associated to `freq_min`. | ||
| # So for a 1 Hz cutoff frequency, the margin will be 5 seconds! | ||
|
|
||
| # %% | ||
| margin_in_s = recording_filt.margin_samples / recording_lfp.sampling_frequency | ||
| print(f"Margin: {margin_in_s} s") | ||
|
|
||
| # %% [markdown] | ||
| # This effectively means that if we plot 1-s snippet of traces, a total of 11 s will actually be read and filtered. Note that the margin can be overridden with the `margin_ms` argument, but we do not recommend changing it. | ||
|
|
||
| # %% | ||
| sw.plot_traces(recording_filt, time_range=[20, 21]) | ||
|
|
||
| # %% [markdown] | ||
| # A warning tells us that what we are doing is not optimized, since in order to get the requested traces the marging "overhead" is very large. | ||
alejoe91 marked this conversation as resolved.
Outdated
Show resolved
Hide resolved
|
||
| # | ||
| # If we ask or plot longer snippets, the warning is not displayed. | ||
|
|
||
| # %% | ||
| sw.plot_traces(recording_filt, time_range=[20, 80]) | ||
|
|
||
| # %% [markdown] | ||
| # ## 4. Quantification and visualization the artifacts | ||
| # | ||
| # Let's extract the traces and visualize the differences between chunking strategies. | ||
| # We'll focus on the chunk boundaries where artifacts appear. | ||
|
|
||
| # %% | ||
| margins_ms = [100, 1000, 5000] | ||
| chunk_durations = ["1s", "10s", "30s"] | ||
|
|
||
| # %% [markdown] | ||
| # The best we can do is to save the full recording in one chunk. This will cause no artifacts and chunking effects, but in practice it's not possible due to the duration and number of channels of most setups. | ||
| # | ||
| # Since in this toy case we have a single channel 5-min recording, we can use this as "optimal". | ||
|
|
||
| # %% | ||
| recording_optimal = recording_filt.save(format="memory", chunk_duration="1000s") | ||
|
|
||
| # %% | ||
| recording_optimal | ||
|
|
||
| # %% [markdown] | ||
| # Now we can do the same with our various options: | ||
|
|
||
| # %% | ||
| recordings_chunked = {} | ||
|
|
||
| for margin_ms in margins_ms: | ||
| for chunk_duration in chunk_durations: | ||
| print(f"Margin ms: {margin_ms} - Chunk duration: {chunk_duration}") | ||
| t_start = time.perf_counter() | ||
| recording_chunk = spre.bandpass_filter( | ||
| recording_with_lfp, | ||
| freq_min=1.0, | ||
| freq_max=300.0, | ||
| margin_ms=margin_ms, | ||
| ignore_low_freq_error=True | ||
| ) | ||
| recording_chunk = recording_chunk.save( | ||
| format="memory", | ||
| chunk_duration=chunk_duration, | ||
| verbose=False, | ||
| ) | ||
| t_stop = time.perf_counter() | ||
| result_dict = { | ||
| "recording": recording_chunk, | ||
| "time": t_stop - t_start | ||
| } | ||
| recordings_chunked[(margin_ms, chunk_duration)] = result_dict | ||
|
|
||
| # %% [markdown] | ||
| # Let's visualize the error for the "10s" chunks and different margins, centered around 30s (which is a chunk edge): | ||
|
|
||
| # %% | ||
| fig, ax = plt.subplots(figsize=(10, 5)) | ||
| trace_plotted = False | ||
| for recording_key, recording_dict in recordings_chunked.items(): | ||
| recording_chunk = recording_dict["recording"] | ||
| margin, chunk = recording_key | ||
| start_frame = int(25 * recording_optimal.sampling_frequency) | ||
| end_frame = int(35 * recording_optimal.sampling_frequency) | ||
| traces_opt = recording_optimal.get_traces(start_frame=start_frame, end_frame=end_frame) | ||
| if not trace_plotted: | ||
| ax.plot(traces_opt, color="grey", label="traces", alpha=0.5) | ||
| trace_plotted = True | ||
| if chunk != "10s": | ||
| continue | ||
| diff = recording_optimal - recording_chunk | ||
| traces_diff = diff.get_traces(start_frame=start_frame, end_frame=end_frame) | ||
| ax.plot(traces_diff, label=recording_key) | ||
alejoe91 marked this conversation as resolved.
Outdated
Show resolved
Hide resolved
|
||
|
|
||
| ax.legend() | ||
|
|
||
| # %% [markdown] | ||
| # For smaller chunk sizes, these artifact will happen more often. In addition, the margin "overhead" will make processing slower. | ||
alejoe91 marked this conversation as resolved.
Outdated
Show resolved
Hide resolved
|
||
| # Let's quantify these concepts by computing the overall absolute error with respect to the optimal case and processing time. | ||
|
|
||
| # %% | ||
| trace_plotted = False | ||
| traces_optimal = recording_optimal.get_traces() | ||
| data = {"margin": [], "chunk": [], "error": [], "time": []} | ||
| for recording_key, recording_dict in recordings_chunked.items(): | ||
| recording_chunk = recording_dict["recording"] | ||
| time = recording_dict["time"] | ||
| margin, chunk = recording_key | ||
| traces_chunk = recording_chunk.get_traces() | ||
| error = np.sum(np.abs(traces_optimal - traces_chunk)) | ||
| data["margin"].append(margin) | ||
| data["chunk"].append(chunk) | ||
| data["error"].append(error) | ||
| data["time"].append(time) | ||
|
|
||
| df = pd.DataFrame(data=data) | ||
|
|
||
| # %% | ||
| fig, axs = plt.subplots(ncols=2, figsize=(10, 5)) | ||
| sns.barplot(data=data, x="margin", y="error", hue="chunk", ax=axs[0]) | ||
| axs[0].set_yscale("log") | ||
| sns.barplot(data=data, x="margin", y="time", hue="chunk", ax=axs[1]) | ||
| axs[0].set_title("Error VS margin x chunk size") | ||
| axs[1].set_title("Processing time VS margin x chunk size") | ||
|
|
||
|
|
||
| sns.despine(fig) | ||
|
|
||
| # %% [markdown] | ||
| # ## 4. Summary | ||
| # | ||
| # 1. **Low-frequency filters require special care**: Filters with low cutoff frequencies (< 10 Hz) have long | ||
| # impulse responses that require large margins to avoid edge artifacts. | ||
| # | ||
| # 2. **Chunking artifacts are real**: When processing data in chunks, insufficient margins lead to visible | ||
| # discontinuities and errors at chunk boundaries. | ||
| # | ||
| # 3. **The solution: large chunks and large margins**: For LFP extraction (1-300 Hz), use: | ||
| # - Chunk size: 30-60 seconds | ||
| # - Margin size: 5 seconds (for 1 Hz highpass) (**use defaults!**) | ||
| # - This is less memory-efficient but more accurate | ||
| # | ||
| # 4. **Downsample after filtering**: After bandpass filtering, downsample to reduce data size (e.g., to 1-2.5 kHz | ||
| # for 300 Hz max frequency). | ||
| # | ||
| # 5. **Trade-offs**: There's always a trade-off between computational efficiency (smaller chunks, less memory) | ||
| # and accuracy (larger chunks, fewer artifacts). For LFP analysis, accuracy should take priority. | ||
| # | ||
| # **When processing your own data:** | ||
| # - If you have memory constraints, use the largest chunk size your system can handle | ||
| # - Always verify your filtering parameters on a small test segment first | ||
| # - Consider the lowest frequency component you want to preserve when setting margins | ||
| # - Save the processed LFP data to disk to avoid recomputing | ||
This file contains hidden or bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Oops, something went wrong.
Oops, something went wrong.
Add this suggestion to a batch that can be applied as a single commit.
This suggestion is invalid because no changes were made to the code.
Suggestions cannot be applied while the pull request is closed.
Suggestions cannot be applied while viewing a subset of changes.
Only one suggestion per line can be applied in a batch.
Add this suggestion to a batch that can be applied as a single commit.
Applying suggestions on deleted lines is not supported.
You must change the existing code in this line in order to create a valid suggestion.
Outdated suggestions cannot be applied.
This suggestion has been applied or marked resolved.
Suggestions cannot be applied from pending reviews.
Suggestions cannot be applied on multi-line comments.
Suggestions cannot be applied while the pull request is queued to merge.
Suggestion cannot be applied right now. Please check back later.
Uh oh!
There was an error while loading. Please reload this page.