Skip to content

Conversation

@sebriq
Copy link

@sebriq sebriq commented Feb 27, 2018

Hello, in the past I changed the code of the dekupl-run Snakefile and dekupl-annotation to use it on ensembl official gff/gtf, making possible to run de-kupl on non-human models. My initial tests were based on the zebrafish annotation for example. I tried to make this version with the minimum of modifications and exclusively on dekupl-run. To be brief the genes names and other informations are stored differentially in the fasta and I changed the extraction in consequence. The config file contain now the "annotation_type" that can be "gencode" or "ensembl", it works as an option. Finally the gff file of ensembl don't contain the ENSGXXXXXXXX .X information, so I removed it when the gene reference is extracted form the fasta. Otherwise dekupl-annotation getSwitches.R code will output nothing. DEKUPL-ANNOTATION IS NOT FULLY TESTED WITH THIS VERSION. I'm afraid that i must remove the .X information in transcript references too, even if I'm not sure that transcript reference is used in gff management of dekupl-annotation.

ps : it lack a space in annotation download commande, do not accept this modification.

@sebriq sebriq changed the title modify Snakefile and add option in conf file to run dekupl on gencod… modify Snakefile and add option in conf file to run dekupl on ensembl… Feb 27, 2018
@jaudoux
Copy link
Collaborator

jaudoux commented Mar 12, 2018

Hi @sebriq, I had not seen the pull request. It is a good idea, thanks. However, could you update the README with the informations regarding the new option before I merge the two branches?

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment

Labels

None yet

Projects

None yet

Development

Successfully merging this pull request may close these issues.

2 participants