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FURIN stimulates NOTCH2 and NOTCH3 Pathways Leading to Return of Function in Aged Muscle Cells

This repository contains the codes for the original analysis used in paper "FURIN stimulates NOTCH2 and NOTCH3 Pathways Leading to Return of Function in Aged Muscle Cells".

Purpose

This Github Repo exists for the purpose of giving individuals the ability to reproduce the findings and figures found in the previously named paper. All code was written by Jiaxing Liu and as a result any issues with the Github should be directed towards him via issues or email correspodence.

Data Availability

The raw datasets analyzed in this study are publicly available through the Genotype-Tissue Expression (GTEx) Project (https://gtexportal.org/). "GTEx V8 Gene read counts for Skin - Sun Exposed (Lower leg)" and "GTEx V8 Gene read counts for Skin - Not Sun Exposed (Suprapubic)" datasets are required prior to running. Both datasets can be accessed via the GTEx Portal or via our UBox cloud storage upon request.

Content

This repository include R notebook with the codes for the in-silica analysis and the results.

  • Main_Code:
    • Project_GIT.RMD: Main code for analysis and visualization used for the paper.
    • Molecules.csv: List of molecules investigated in the analysis.
  • Results: Results and visualization that are generated with main codes and displayed in the paper.
    • Skin_lower_leg: Results and visualization for "GTEx V8 Gene read counts for Skin - Sun Exposed (Lower leg)" data.
    • Skin_suprapubic: Results and visualization for "GTEx V8 Gene read counts for Skin - Not Sun Exposed (Suprapubic)" data.

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