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Modeling development of tertiary lymphoid structures in pulmonary tuberculosis by 3D profiling and trajectory analysis


TABLE OF CONTENTS

  • GENERAL INFORMATION
    • ASSOCIATED PUBLICATION
    • RECOMMENDED CITATION
    • USEFUL LINKS
  • ACCESS THE DATASET
    • FILE ORGANIZATION
    • REPOSITORY LINKS
    • FILE LIST
  • ADDITIONAL NOTES/COMMENTS ​

GENERAL INFORMATION


Modeling development of tertiary lymphoid structures in pulmonary tuberculosis by 3D profiling and trajectory analysis

Authors: Emmanuel C. Ogbonna*, Soheil R. Talemi*, Zoltan Maliga, Ziyuan Zhao, Clarence Yapp, Shishir Pant, Yi Daniel Lu, Jeremy Muhlich, Andréanne Gagné, Amanda J. Martinot, Shannon Coy, Sandro Santagata, Angela Shih, Isaac H. Solomon, Threnesan Naidoo, Bree B. Aldridge, Adrie Steyn, Peter K. Sorger†

*Authors contributed equally to this article.
†Corresponding.

Please cite this data as the following:

Ogbonna and Rastgou Talemi (2026). Modeling development of tertiary lymphoid structures in pulmonary tuberculosis by 3D profiling and trajectory analysis. biorxiv https://doi.org/10.64898/2026.XX.XX.725201 {journal/biorxv}

Relevant links:


ACCESS THE DATASET

File organization:

Each file follows the following naming convention:
​ Each folder corresponds to a patient sample (N).

File Type Description Location
N.ome.tif Stitched multiplex CyCIF image pyramid in ome.tif format AWS
N.ome.tif Hematoxylin and Eosin stained image of adjacent FFPE tissue section AWS
N-cellRing. ome.tif Segmentation mask image AWS
N.csv single-cell feature table, including intensity data for all channels AWS
N.fastq GeoMx Sequencing data GEO
N.ims Stitched and registered 3D multiplexed CyCIF image in .ims format AWS

AWS Data Access

​ Full-resolution CyCIF images, single cell segmentation masks, and cell count tables will be available via AWS.​ You will need the following bucket name: ​

AWS BUCKET NAME  

For general instructions on how to download data from AWS, see: https://zenodo.org/records/10223574

If you experience issues accessing the above AWS S3 buckets, email tissue-atlas(at)hms.harvard.edu with the subject line "bucketname: Data Access".

GEO Data Access

​ GeoMx gene expression data is available via the Gene Expression Omnibus (GEO): Accession number:  GSE318959 ​

Other Repository

Image data generated during this study are available on the Gates TB Data Portal (https://idp.tissue-atlas.org/). For access, email tissue-atlas(at)hms.harvard.edu with the subject line "Gates TB Data Portal: Data Access".

FILE LIST

CyCIF Image Data

Patient ID File Name Location File Size (GB) Antibody Panel
Patient1_B4 LSP24522.ome.tif AWS 222.37 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24525.ome.tif AWS 202.51 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24528.ome.tif AWS 202.57 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24531.ome.tif AWS 225.94 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24534.ome.tif AWS 220.79 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24537.ome.tif AWS 204.92 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24540.ome.tif AWS 191.57 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24543.ome.tif AWS 213.09 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24546.ome.tif AWS 205.37 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24549.ome.tif AWS 212.91 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24552.ome.tif AWS 199.53 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24555.ome.tif AWS 212.91 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24558.ome.tif AWS 221.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24561.ome.tif AWS 217.61 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24564.ome.tif AWS 214.29 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24567.ome.tif AWS 196.63 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24570.ome.tif AWS 207.13 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24573.ome.tif AWS 226.64 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24576.ome.tif AWS 230.50 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24579.ome.tif AWS 205.65 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24582.ome.tif AWS 226.38 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24585.ome.tif AWS 199.04 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24588.ome.tif AWS 215.38 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24591.ome.tif AWS 200.37 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24594.ome.tif AWS 217.11 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24597.ome.tif AWS 199.92 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24600.ome.tif AWS 202.57 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24603.ome.tif AWS 219.74 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24606.ome.tif AWS 219.70 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24609.ome.tif AWS 226.05 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24612.ome.tif AWS 197.44 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24615.ome.tif AWS 222.30 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24618.ome.tif AWS 197.84 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24621.ome.tif AWS 198.85 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24624.ome.tif AWS 198.49 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24627.ome.tif AWS 216.52 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24630.ome.tif AWS 210.79 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24633.ome.tif AWS 193.94 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24636.ome.tif AWS 203.49 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24639.ome.tif AWS 185.99 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B5 LSP16101.ome.tif AWS 214.00 ORION imaging panel
Patient1_B5 LSP16102_B5_Scan1.ome.tiff AWS 15.30 p17_e6_TB_Granulomas_GeoMx IF
Patient1_B5 LSP16102.ome.tif AWS 19.20 EO8_p17e25_PostGeoMx_CyCIF
Patient1_B5 LSP16104.ome.tif AWS 147.00 EO4_TB_MDSC_rerun
Patient1_B5 LSP16105.ome.tif AWS 164.00 EO5_B5 and AHRI samples
Patient2 LSP16031.ome.tif AWS 11.30 p17_e6_TB_Granulomas_GeoMx IF

H&E Image Data

Patient ID File Name Location File Size (GB)
Patient1_B4 LSP24521.ome.tif AWS 12.15
Patient1_B4 LSP24524.ome.tif AWS 14.00
Patient1_B4 LSP24527.ome.tif AWS 13.48
Patient1_B4 LSP24530.ome.tif AWS 12.78
Patient1_B4 LSP24533.ome.tif AWS 13.78
Patient1_B4 LSP24536.ome.tif AWS 16.61
Patient1_B4 LSP24539.ome.tif AWS 16.72
Patient1_B4 LSP24542.ome.tif AWS 16.42
Patient1_B4 LSP24545.ome.tif AWS 18.33
Patient1_B4 LSP24548.ome.tif AWS 13.97
Patient1_B4 LSP24551.ome.tif AWS 12.54
Patient1_B4 LSP24554.ome.tif AWS 13.91
Patient1_B4 LSP24557.ome.tif AWS 13.83
Patient1_B4 LSP24560.ome.tif AWS 13.21
Patient1_B4 LSP24563.ome.tif AWS 13.25
Patient1_B4 LSP24566.ome.tif AWS 13.93
Patient1_B4 LSP24569.ome.tif AWS 13.46
Patient1_B4 LSP24572.ome.tif AWS 14.41
Patient1_B4 LSP24575.ome.tif AWS 13.23
Patient1_B4 LSP24578.ome.tif AWS 13.43
Patient1_B4 LSP24581.ome.tif AWS 13.23
Patient1_B4 LSP24584.ome.tif AWS 12.64
Patient1_B4 LSP24587.ome.tif AWS 13.73
Patient1_B4 LSP24590.ome.tif AWS 13.01
Patient1_B4 LSP24593.ome.tif AWS 12.73
Patient1_B4 LSP24596.ome.tif AWS 13.72
Patient1_B4 LSP24599.ome.tif AWS 17.65
Patient1_B4 LSP24602.ome.tif AWS 11.89
Patient1_B4 LSP24605.ome.tif AWS 16.95
Patient1_B4 LSP24608.ome.tif AWS 14.84
Patient1_B4 LSP24611.ome.tif AWS 13.68
Patient1_B4 LSP24614.ome.tif AWS 13.78
Patient1_B4 LSP24617.ome.tif AWS 13.49
Patient1_B4 LSP24620.ome.tif AWS 14.22
Patient1_B4 LSP24623.ome.tif AWS 13.12
Patient1_B4 LSP24626.ome.tif AWS 13.01
Patient1_B4 LSP24629.ome.tif AWS 13.53
Patient1_B4 LSP24632.ome.tif AWS 12.78
Patient1_B4 LSP24635.ome.tif AWS 13.39
Patient1_B4 LSP24638.ome.tif AWS 11.72
Patient1_B5 LSP16103.ome.tif AWS 0.84
Patient1_B5 LSP16106.ome.tiff AWS 19.70

Segmentation Mask Data

Patient ID File Name File Size (GB) Antibody Panel
Patient1_B4 LSP24522.ome.tif 17.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24525.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24528.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24531.ome.tif 17.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24534.ome.tif 17.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24537.ome.tif 16.73 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24540.ome.tif 14.93 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24543.ome.tif 16.73 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24546.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24549.ome.tif 17.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24552.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24555.ome.tif 16.84 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24558.ome.tif 17.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24561.ome.tif 17.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24564.ome.tif 16.84 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24567.ome.tif 15.57 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24570.ome.tif 16.84 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24573.ome.tif 18.05 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24576.ome.tif 18.05 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24579.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24582.ome.tif 18.05 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24585.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24588.ome.tif 17.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24591.ome.tif 15.57 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24594.ome.tif 16.73 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24597.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24600.ome.tif 16.84 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24603.ome.tif 17.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24606.ome.tif 17.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24609.ome.tif 18.05 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24612.ome.tif 16.08 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24615.ome.tif 18.18 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24618.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24621.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24624.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24627.ome.tif 17.39 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24630.ome.tif 16.73 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24633.ome.tif 15.57 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24636.ome.tif 16.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24639.ome.tif 14.93 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B5 LSP16101.ome.tif 22.30 ORION imaging panel
Patient1_B5 LSP16102.ome.tif 0.316 EO8_p17e25_PostGeoMx_CyCIF
Patient1_B5 LSP16104.ome.tif 6.83 EO4_TB_MDSC_rerun
Patient1_B5 LSP16105.ome.tif 7.16 EO5_B5 and AHRI samples

Features Table Data

Patient ID File Name File Size (GB) Antibody Panel
Patient1_B4 LSP24522.csv 1.16 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24525.csv 1.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24528.csv 1.16 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24531.csv 1.31 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24534.csv 1.18 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24537.csv 1.19 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24540.csv 1.21 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24543.csv 1.21 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24546.csv 1.23 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24549.csv 1.19 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24552.csv 1.22 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24555.csv 1.18 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24558.csv 1.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24561.csv 1.14 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24564.csv 1.17 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24567.csv 1.16 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24570.csv 1.30 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24573.csv 1.13 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24576.csv 1.13 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24579.csv 1.10 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24582.csv 1.16 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24585.csv 1.11 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24588.csv 1.13 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24591.csv 1.11 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24594.csv 1.14 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24597.csv 1.07 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24600.csv 1.10 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24603.csv 1.13 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24606.csv 1.08 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24609.csv 1.11 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24612.csv 0.99 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24615.csv 1.20 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24618.csv 0.96 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24621.csv 0.98 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24624.csv 0.96 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24627.csv 1.00 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24630.csv 0.98 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24633.csv 0.94 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24636.csv 1.00 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B4 LSP24639.csv 0.92 p17e16_EO7_B4_3D IMAGE RECONSTRUCTION
Patient1_B5 LSP16101.csv 0.51 ORION imaging panel
Patient1_B5 LSP16102.csv 1.67 EO8_p17e25_PostGeoMx_CyCIF
Patient1_B5 LSP16104.csv 1.71 EO4_TB_MDSC_rerun
Patient1_B5 LSP16105.csv 2.24 EO5_B5 and AHRI samples

3D Image Data (in Imaris Format)

Patient ID File Name Location File Size (GB)
Patient1_B4 Patient1_B4_3D_TLS.ims AWS 8640.00
Patient1_B4 output1.ims AWS 124.00
Patient1_B4 output2.ims AWS 72.70
Patient1_B4 output4.ims AWS 65.60
Patient1_B4 output5.ims AWS 142.00
Patient1_B4 output6.ims AWS 65.30

ADDITIONAL NOTES/COMMENTS

​ ​Please contact tissue-atlas(at)hms.harvard.edu if you have any questions related to the data.

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Multidimensional Spatial Characterization of Tertiary Lymphoid Structures in Human Pulmonary TB Lung Granulomas

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