Issues

• NCBI BLAST accession wrappers prevent associated FASTA realignment

  light-suzuki/primerblast-oss#72 · Filter by author light-suzuki opened on Oct 2, 2026

• [P1][Genotyping] Define gel-aware multi-reference acceptance for AS-PCR and tetra-ARMS

  light-suzuki/primerblast-oss#68 · Filter by author light-suzuki opened on Oct 2, 2026

• [P1][Multi-reference] Separate target conservation from same-size off-locus amplification

  light-suzuki/primerblast-oss#67 · Filter by author light-suzuki opened on Oct 2, 2026

• [P1][Reliability] Do not convert Primer3/runtime failures into silent tiling gaps

  light-suzuki/primerblast-oss#66 · Filter by author light-suzuki opened on Oct 2, 2026

• [P0][Validation] Make wet-lab validation band-level, gel-aware, and prediction-immutable

  light-suzuki/primerblast-oss#65 · Filter by author light-suzuki opened on Oct 2, 2026

• Performance: stream/index large VCF inputs and keep genome-scale memory bounded

  light-suzuki/primerblast-oss#46 · Filter by author light-suzuki opened on Sep 27, 2026

• [P1][Calibration] Refit rank/risk scoring from wet-lab outcomes once the validation panel is populated

  light-suzuki/primerblast-oss#35 · Filter by author light-suzuki opened on Sep 26, 2026

• [P1][Performance] Batch primer BLAST queries per database instead of spawning one process per primer

  light-suzuki/primerblast-oss#34 · Filter by author light-suzuki opened on Sep 26, 2026
  2

• [P0][Validation] Populate the prospective wet-lab panel and calibrate specificity/risk predictions

  light-suzuki/primerblast-oss#33 · Filter by author light-suzuki opened on Sep 26, 2026