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Update readme.md binding lifetime #7

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4 changes: 2 additions & 2 deletions Analysis/Protein binding lifetime from kymograph/readme.md
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<b>Author</b>: Aafke van den Berg<br>
<b>Research subjects</b>: DNA-binding proteins<br>
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<p>This Notebook contains a full example analysis workflow for determining the duration of binding of a protein to DNA. The binding lifetime is determined by tracking the duration of binding events of a fluorescently labeled protein on a kymograph, as illustrated in the figure below. The durations are then collected and fitted to an exponential distribution to determine the binding lifetime.
<p>This Notebook contains a full example analysis workflow for determining the binding lifetime of a protein to DNA. The binding lifetime is determined by tracking the duration of binding events of a fluorescently labeled protein on a kymograph, as illustrated in the figure below. The durations are then collected and fitted to an exponential distribution to determine the binding lifetime. The binding lifetime relates to the off rate as k<sub>off</sub> = 1/binding lifetime.
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</em></p><p><br></p><div class="se-component se-image-container __se__float-none"><figure style="margin: 0px;"><img style="" data-origin="," data-file-size="31661" data-file-name="binding_lifetime.PNG" data-percentage="auto,auto" data-align="none" data-size="," data-rotatey="" data-rotatex="" data-proportion="true" data-rotate="" alt="" src="binding_lifetime.PNG" data-index="3"></figure></div><p><em>Figure 1: The duration of binding can be determined from a kymograph by looking at the length of a binding event.</em></p><p><br></p><p><em>
</em></p><p><br></p><div class="se-component se-image-container __se__float-none"><figure style="margin: 0px;"><img style="" data-origin="," data-file-size="31661" data-file-name="binding_lifetime.PNG" data-percentage="auto,auto" data-align="none" data-size="," data-rotatey="" data-rotatex="" data-proportion="true" data-rotate="" alt="" src="binding_lifetime.PNG" data-index="3"></figure></div><p><em>Figure 1: A kymograph with a protein labeled in green binding and unbinding from DNA. The duration of binding can be determined from a kymograph by looking at the length of a binding event.</em></p><p><br></p><p><em>
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