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  1. Subtilisin_ProteinMPNN_DataAndCode Subtilisin_ProteinMPNN_DataAndCode Public

    线虫捕食性真菌可通过分泌多种胞外水解酶降解线虫体壁相关组分，因而在生物防治与酶工程领域具有较高的潜在应用价值。然而，针对目标酶开展序列层面的理性设计时，如何在维持催化相关结构与功能特征的前提下实现理化性质优化，仍面临功能保守性与性质改良之间的权衡。基于此，本研究构建了一套融合进化约束与结构感知深度学习的酶序列设计与层次化计算筛选流程，旨在提高候选突变体在保持功能相关特征前提下实现理化性质优化…

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